The Scanning Cryomacroscope - A Device Prototype for the Study of Cryopreservation.
The Scanning Cryomacroscope - A Device Prototype for the Study of Cryopreservation.
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DOI：
10.1016/j.cryogenics.2014.04.017
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发表时间：
2014-07
期刊：
影响因子：
2.1
通讯作者：
Rabin, Yoed
中科院分区：
文献类型：
--
作者：
Feig, Justin S. G.;Rabin, Yoed
本文介绍了一种新的低温宏观镜原型--一种用于低温保存生物样品原位分析的可视化装置。为了使大于光学装置视场的样品可视化，扫描机构被集成到系统中，这代表了对以前的低温宏观镜原型的关键改进。新设计的另一个关键特点是与现有的顶部加载控制速率冷却室兼容，从而消除了对专用冷却机构的需求。当前开发的目标是创建生成实验研究的单个数字电影的方法，其中所有相关数据都被覆盖。扫描低温显微镜的可视化能力在当前对冷冻保护剂二甲基亚砜和冷冻保护鸡尾酒DP 6的研究中得到了证明。所展示的效应包括玻璃形成、各种结晶制度、热收缩和断裂形成。
A new cryomacroscope prototype—a visualization device for the in situ analysis of cryopreserved biological samples—is presented in the current study. In order to visualize samples larger than the field of view of the optical setup, a scanning mechanism is integrated into the system, which represents a key improvement over previous cryomacroscope prototypes. Another key feature of the new design is in its compatibility with available top-loading controlled-rate cooling chambers, which eliminates the need for a dedicated cooling mechanism. The objective for the current development is to create means to generate a single digital movie of an experimental investigation, with all relevant data overlaid. The visualization capabilities of the scanning cryomacroscope are demonstrated in the current study on the cryoprotective agent dimethyl sulfoxide and the cryoprotective cocktail DP6. Demonstrated effects include glass formation, various regimes of crystallization, thermal contraction, and fracture formation.
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影响因子：
2.7
作者：
FAHY, GM;SAUR, J;WILLIAMS, RJ
通讯作者：
WILLIAMS, RJ
影响因子：
2.7
作者：
Pegg, DE;Wusteman, MC;Boylan, S
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作者：
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通讯作者：
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作者：
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作者：
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通讯作者：
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