Strontium-releasing fluorapatite glass-ceramic scaffolds: Structural characterization and in vivo performance.
Strontium-releasing fluorapatite glass-ceramic scaffolds: Structural characterization and in vivo performance.
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DOI：
10.1016/j.actbio.2018.05.047
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发表时间：
2018-07-15
影响因子：
9.7
通讯作者：
Holloway JA
中科院分区：
文献类型：
--
作者：
Denry I;Goudouri OM;Fredericks DC;Akkouch A;Acevedo MR;Holloway JA
在骨组织工程中，生物可降解陶瓷支架能够原位输送有利于骨形成的离子物种，受到越来越多的关注。锶已被证明是成骨的，但在欧洲用于治疗骨质疏松症的含锶药物，如雷奈酸锶，现在由于全身效应的临床证据而受到限制。通过在氟磷灰石基玻璃中掺入锶，我们研制出了具有完全互连的大孔率和与松质骨相似的细胞尺寸的陶瓷支架，它也能够释放锶。用X射线衍射仪和扫描电子显微镜研究了微晶玻璃的析晶行为，发现在无锶微晶玻璃的表面形成了镁铝尖晶石和氟磷灰石，在掺锶的微晶玻璃表面形成了锶取代的氟磷灰石。在植入大鼠颅骨临界大小的缺损区后8周，掺入最高量锶的支架导致最高的矿物沉积率。无锶玻璃陶瓷支架的新生骨数量明显较多，可能与支架表面存在镁铁榴石有关。通过对颅骨切片的能量色散XRF分析，我们证明了掺锶支架在新形成的骨中存在锶，而在新形成的骨中存在大量的氟，无论其成分或结晶状态如何。
There is increasing interest in biodegradable ceramic scaffolds for bone tissue engineering capable of in situ delivery of ionic species favoring bone formation. Strontium has been shown to be osteogenic, but strontium-containing drugs such as strontium ranelate, used in Europe for the treatment of osteoporosis, are now restricted due to clinical evidence of systemic effects. By doping fluorapatite-based glasses with strontium, we developed ceramic scaffolds with fully interconnected macroporosity and cell size similar to that of cancellous bone, that are also capable of releasing strontium. The crystallization behavior, investigated by XRD and SEM, revealed the formation of akermanite and fluorapatite at the surface of strontium-free glass-ceramic scaffolds, and strontium-substituted fluorapatite at the surface of the strontium-doped scaffolds. At 8 weeks after implantation in a rat calvarial critical size defect, scaffolds doped with the highest amount of strontium led to the highest mineral apposition rate. A significantly higher amount of newly-formed bone was found with the strontium-free glass-ceramic scaffold, and possibly linked to the presence of akermanite at the scaffold surface. We demonstrate by energy dispersive XRF analyses of skull sections that strontium was present in newly formed bone with the strontium-doped scaffolds, while a significant amount of fluorine was incorporated in newly formed bone, regardless of composition or crystallization state.
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锶取代硅酸钙生物活性陶瓷增强骨质疏松骨再生
影响因子：
14
作者：
Lin, Kaili;Xia, Lunguo;Chang, Jiang
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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通讯作者：
Kuhn LT
影响因子：
4.1
作者：
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DOI：
10.4028/www.scientific.net/msf.278-281.814
发表时间：
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期刊：
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影响因子：
--
作者：
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通讯作者：
Tedesco, E