Paper biosensors for detecting elevated IL-6 levels in blood and respiratory samples from COVID-19 patients.
Paper biosensors for detecting elevated IL-6 levels in blood and respiratory samples from COVID-19 patients.
复制标题
纸质生物传感器用于检测新冠肺炎患者血液和呼吸道样本中升高的IL-6水平。
DOI：
10.1016/j.snb.2020.129333
复制
发表时间：
2021-03-01
期刊：
影响因子：
--
通讯作者：
de la Rica R
中科院分区：
文献类型：
--
作者：
Adrover-Jaume C;Alba-Patiño A;Clemente A;Santopolo G;Vaquer A;Russell SM;Barón E;González Del Campo MDM;Ferrer JM;Berman-Riu M;García-Gasalla M;Aranda M;Borges M;de la Rica R
分散COVID-19护理减少了传染，并更好地利用了医院资源。我们引入生物传感器，旨在在分散的医疗环境中检测COVID-19的严重病例。它们由与智能手机接口的纸质免疫传感器组成。免疫传感器被设计为当样本含有超低浓度的IL-6时产生强烈的比色信号，IL-6已被提议作为COVID-19的预后生物标志物。这是通过将基于纸张的信号放大机制与用于分配抗体修饰的纳米颗粒的聚合物填充的储器和用于颜色定量的定制应用程序相结合来实现的。通过这种设计，我们在PBS中实现了10−3 pg mL-1的低检测限（LOD）和10−3至102 pg mL-1的宽动态范围内的半定量测量。测定时间在10分钟以下。低LOD使我们能够稀释血液样品并检测IL-6，LOD为1.3 pg mL-1，动态范围高达102 pg mL-1。根据该方案，我们能够根据IL-6的不同血液水平对COVID-19患者进行分层。我们还报告了COVID-19患者呼吸道样本（支气管抽吸物，BAS）中IL-6的检测结果。通过相应地改变修饰纳米颗粒的抗体，该测试可以很容易地适用于检测其他细胞因子，如TNF-α和IL-8。检测血液和呼吸道样本中细胞因子的能力为监测肺部局部炎症以及体内全身炎症水平铺平了道路。
Decentralizing COVID-19 care reduces contagions and affords a better use of hospital resources. We introduce biosensors aimed at detecting severe cases of COVID-19 in decentralized healthcare settings. They consist of a paper immunosensor interfaced with a smartphone. The immunosensors have been designed to generate intense colorimetric signals when the sample contains ultralow concentrations of IL-6, which has been proposed as a prognosis biomarker of COVID-19. This is achieved by combining a paper-based signal amplification mechanism with polymer-filled reservoirs for dispensing antibody-decorated nanoparticles and a bespoken app for color quantification. With this design we achieved a low limit of detection (LOD) of 10−3 pg mL-1 and semi-quantitative measurements in a wide dynamic range between 10−3 and 102 pg mL-1 in PBS. The assay time is under 10 min. The low LOD allowed us to dilute blood samples and detect IL-6 with an LOD of 1.3 pg mL-1 and a dynamic range up to 102 pg mL-1. Following this protocol, we were able to stratify COVID-19 patients according to different blood levels of IL-6. We also report on the detection of IL-6 in respiratory samples (bronchial aspirate, BAS) from COVID-19 patients. The test could be easily adapted to detect other cytokines such as TNF-α and IL-8 by changing the antibodies decorating the nanoparticles accordingly. The ability of detecting cytokines in blood and respiratory samples paves the way for monitoring local inflammation in the lungs as well as systemic inflammation levels in the body.
登录
查看更多内容
影响因子：
9
作者：
Channappanavar R;Perlman S
通讯作者：
Perlman S
影响因子：
3.3
作者：
Aydin, Elif Burcu;Aydin, Muhammet;Sezginturk, Mustafa Kemal
通讯作者：
Sezginturk, Mustafa Kemal
DOI：
10.1016/j.snb.2020.128971
发表时间：
2021-02-01
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Karami A;Hasani M;Azizi Jalilian F;Ezati R
通讯作者：
Ezati R
影响因子：
82.9
作者：
Del Valle DM;Kim-Schulze S;Huang HH;Beckmann ND;Nirenberg S;Wang B;Lavin Y;Swartz TH;Madduri D;Stock A;Marron TU;Xie H;Patel M;Tuballes K;Van Oekelen O;Rahman A;Kovatch P;Aberg JA;Schadt E;Jagannath S;Mazumdar M;Charney AW;Firpo-Betancourt A;Mendu DR;Jhang J;Reich D;Sigel K;Cordon-Cardo C;Feldmann M;Parekh S;Merad M;Gnjatic S
通讯作者：
Gnjatic S
DOI：
10.4269/ajtmh.20-1110
发表时间：
2020-12
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Kwon JS;Kim JY;Kim MC;Park SY;Kim BN;Bae S;Cha HH;Jung J;Kim MJ;Lee MJ;Choi SH;Chung JW;Shin EC;Kim SH
通讯作者：
Kim SH