Systematic analysis of protein expression in Candida albicans exposed to farnesol
Systematic analysis of protein expression in Candida albicans exposed to farnesol
复制标题
法呢醇暴露的白色念珠菌蛋白质表达的系统分析
DOI：
10.1097/cm9.0000000000000420
复制
发表时间：
2019-09
期刊：
影响因子：
--
通讯作者：
Liu Z H
中科院分区：
文献类型：
--
作者：
Wang F J;Liu Z H
Abstract Background: The phenotypic switching of Candida spp. plays an important role in the development of vulvovaginal candidiasis (VVC). Farnesol, as a quorum-sensing molecule in Candida albicans, has the ability to prevent yeast-to-hyphal conversion in vitro. However, the mechanism underlying this ability is unclear. This study aimed to investigate changes in protein levels to better understand how farnesol impacts processes contributing to VVC. Methods: The isobaric tag for relative and absolute quantitation technique was used to detect protein expression in C. albicans strain SC5314 (ATCC MYA-2876) with or without farnesol exposure. Proteins with a threshold fold change greater than 1.5 were screened and considered differentially expressed proteins. All the altered proteins were analyzed using Gene Ontology annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, and metabolic pathway annotation. Results: Between the farnesol-exposed group and the farnesol-unexposd group, we detected 297 altered proteins among all 2047 tested proteins based on a threshold fold change of more than 1.5 (P < 0.05). Eighty-seven of the 297 altered proteins exhibited metabolic enzyme activity and participated in 85 metabolic pathways according to KEGG pathway analysis. Most of these metabolic pathways were associated with central carbon metabolism processes. In the sterol synthesis pathway, which involves the synthesis of farnesol, ERG25 (methylsterol monooxygenase) and ERG4 (delta 24(24(1))-sterol reductase) were both down-regulated in the farnesol-exposed group. All six altered proteases associated with the oxidative phosphorylation process were down-regulated in the farnesol-exposed group relative to the farnesol-unexposed group. Conclusions: The mechanisms underlying farnesol-induced phenotype switching involves the adjustment of metabolic activities and epigenetic modification. Exogenous farnesol had an evident, but non-deterministic effect on the synthesis of ergosterol. The potential drug activity of farnesol warrants further investigation.
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DOI：
10.15698/mic2017.09.589
发表时间：
2017-09-04
期刊：
Microbial cell (Graz, Austria)
影响因子：
--
作者：
Egbe NE;Dornelles TO;Paget CM;Castelli LM;Ashe MP
通讯作者：
Ashe MP
重新审视金合欢醇的群体感应
影响因子：
2.5
作者：
Polke M;Jacobsen ID
通讯作者：
Jacobsen ID
影响因子：
4
作者：
Monteiro, D. R.;Arias, L. S.;Delbem, A. C. B.
通讯作者：
Delbem, A. C. B.
影响因子：
3.4
作者：
Sebaa, Sarra;Boucherit-Otmani, Zahia;Courtois, Philippe
通讯作者：
Courtois, Philippe
DOI：
10.1016/j.ijantimicag.2017.09.002
发表时间：
2018-03-01
影响因子：
10.8
作者：
Wiederhold, Nathan P.
通讯作者：
Wiederhold, Nathan P.