S-PRG Filler Eluate Induces Oxidative Stress in Oral Microorganism: Suppression of Growth and Pathogenicity, and Possible Clinical Application.
S-PRG Filler Eluate Induces Oxidative Stress in Oral Microorganism: Suppression of Growth and Pathogenicity, and Possible Clinical Application.
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DOI：
10.3390/antibiotics10070816
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发表时间：
2021-07-05
期刊：
影响因子：
--
通讯作者：
Imai K
中科院分区：
文献类型：
--
作者：
Kono Y;Tamura M;Cueno ME;Tonogi M;Imai K
据认为，控制口腔微生物菌群不仅可以预防口腔疾病，还可以预防由口腔疾病引起的全身性疾病。本研究确立了新型生物活性物质“S-PRG填充剂”对口腔细菌的抗菌作用。我们检查了口腔细菌细胞中 S-PRG 填充剂洗出液中释放的六种离子引起的氧化应激状态。此外，我们研究了这些离子对革兰氏阳性和革兰氏阴性细菌的生长和致病性的影响。我们发现释放的离子影响细菌细胞中的 SOD 含量和过氧化氢，暗示氧化应激的发生。在细菌培养中，根据培养基中的离子浓度观察到生长抑制。此外，释放的离子以浓度依赖性方式抑制变形链球菌对羟基磷灰石的粘附、口腔链球菌神经氨酸酶活性以及牙龈卟啉单胞菌血凝和牙龈蛋白酶活性。从这些结果来看，S-PRG 填充剂释放的离子可以抑制口腔细菌菌群的生长和致病性。这种生物活性材料可能有助于预防口腔内外疾病的发生，进而可能应用于口腔护理和改善生活质量。
Controlling the oral microbial flora is putatively thought to prevent not only oral diseases, but also systemic diseases caused by oral diseases. This study establishes the antibacterial effect of the novel bioactive substance “S-PRG filler” on oral bacteria. We examined the state of oxidative stress caused by the six types of ions released in eluate from the S-PRG filler in oral bacterial cells. Moreover, we investigated the effects of these ions on the growth and pathogenicity of Gram-positive and Gram-negative bacteria. We found that the released ions affected SOD amount and hydrogen peroxide in bacterial cells insinuating oxidative stress occurrence. In bacterial culture, growth inhibition was observed depending on the ion concentration in the medium. Additionally, released ions suppressed Streptococcus mutans adhesion to hydroxyapatite, S. oralis neuraminidase activity, and Porphyromonas gingivalis hemagglutination and gingipain activity in a concentration-dependent manner. From these results, it was suggested that the ions released from the S-PRG filler may suppress the growth and pathogenicity of the oral bacterial flora. This bioactive material is potentially useful to prevent the onset of diseases inside and outside of the oral cavity, which in turn may have possible applications for oral care and QOL improvement.
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影响因子：
8
作者：
COLMAN, PM
通讯作者：
COLMAN, PM
影响因子：
--
作者：
Eick, S.;Reissmann, A.;Pfister, W.
通讯作者：
Pfister, W.
影响因子：
2.6
作者：
D'Aiuto, F.;Gable, D.;Gallagher, J. E.
通讯作者：
Gallagher, J. E.
影响因子：
1.9
作者：
Kaga, Masayuki;Kakuda, Shinichi;Sano, Hidehiko
通讯作者：
Sano, Hidehiko
DOI：
10.2478/v10136-012-0009-8
发表时间：
2012-01-01
期刊：
JOURNAL OF APPLIED BIOMEDICINE
影响因子：
--
作者：
Jebahi, Samira;Oudadesse, Hassane;el Feki, Abdelfattah
通讯作者：
el Feki, Abdelfattah