Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo.
Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo.
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DOI：
10.1038/s41598-017-18365-z
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发表时间：
2017-12-20
影响因子：
4.6
通讯作者：
Wakayama T
中科院分区：
文献类型：
--
作者：
Nagatomo H;Yao T;Araki Y;Mizutani E;Wakayama T
没有透明带（ZP）的卵母细胞通常是由于先天性或操作性影响而出现，但它们难以处理，并且胚胎存活率较低。因此，此类无透明带（ZF）卵母细胞不用于诊所或实验室。此外，在实验室中，通过病毒感染进行基因操作或通过卵裂球分离进行双胞胎生产通常需要去除 ZP，但已有报道对发育产生不利影响。因此，如果胚胎能够被类似于 ZP 的结构所覆盖，那将是非常有价值的。在这项研究中，我们试图确定琼脂糖胶囊是否可以作为 ZP 的替代品。我们的结果表明，来自这些琼脂糖胶囊的胚胎能够正常发育，并且可以移植以获得可存活的后代，而无需去除琼脂糖胶囊。此外，在压实之前，琼脂糖胶囊胚胎表现出良好的冷冻耐受性，与ZF胚胎相比，存活率极高。因此，琼脂糖胶囊是在临床和家畜环境中利用缺乏 ZP 的卵母细胞和胚胎的宝贵工具。
Oocytes without a zona pellucida (ZP) often occur as a result of congenital or operational effects, but they are difficult to handle, and embryonic survival is low. Such zona-free (ZF) oocytes are therefore not used in clinics or laboratories. Furthermore, in the laboratory, removal of the ZP is often necessary for genetic manipulation by viral infection or twin production by blastomere separation, but adverse effects on development have been reported. It would therefore be extremely valuable if the embryo could be covered with a structure similar to that of the ZP. In this study, we sought to determine whether an agarose capsule could serve as a substitute for the ZP. Our results indicate that embryos derived from these agarose capsules were able to develop normally, and could be transplanted to obtain viable offspring, without having to remove the agarose capsule. Furthermore, before compaction, the agarose capsule embryos exhibited good freezing tolerance, and survival rate was extremely high compared to ZF embryos. Thus, agarose capsules represent a valuable tool for utilizing oocytes and embryos that lack a ZP, both in a clinical and livestock setting.
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DOI：
10.1530/jrf.0.0220129
发表时间：
1970-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BRONSON, RA;MCLAREN, A
通讯作者：
MCLAREN, A
DOI：
10.1530/jrf.0.0690315
发表时间：
1983-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
TSUNODA, Y;MCLAREN, A
通讯作者：
MCLAREN, A
影响因子：
3.6
作者：
KIMURA, Y;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R
影响因子：
2.8
作者：
LEHNJENSEN, H;RALL, WF
通讯作者：
RALL, WF
影响因子：
3.6
作者：
CHATOT, CL;LEWIS, JL;ZIOMEK, CA
通讯作者：
ZIOMEK, CA