Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation.
Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation.
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DOI：
10.1016/j.bios.2018.02.040
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发表时间：
2018-06-15
影响因子：
12.6
通讯作者：
Shin S
中科院分区：
文献类型：
--
作者：
Na W;Nam D;Lee H;Shin S
迫切需要在护理点快速筛查和隔离病毒感染患者与具有类似症状的患者。在这项研究中，我们介绍了一种新的微流控方法，用于检测各种病毒，使用滚环扩增（RCA）的病原体的表面上的数千个微珠包装在微通道。当靶向病原体遇到相应的特定模板时，DNA通过RCA过程迅速扩增成特定的哑铃形状，形成DNA水凝胶并阻断珠子之间形成的流动路径。由于反应表面积的显著增加，检测时间缩短到15 min以内，各种病原体的检测限已达到0.1 pM。通过注入染色液体，可以用肉眼确定样品流体中目标病原体的存在。此外，通过整合多通道设计，可以同时对各种感染性病原体（即，埃博拉病毒、中东呼吸综合征（MERS）和其他）。
There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15 min and the detection limit of various pathogens has been reached to 0.1 pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.
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