Recovery of aging-related size increase of skin epithelial cells: in vivo mouse and in vitro human study.
Recovery of aging-related size increase of skin epithelial cells: in vivo mouse and in vitro human study.
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DOI：
10.1371/journal.pone.0122774
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Woodworth CD
中科院分区：
文献类型：
--
作者：
Sokolov I;Guz NV;Iyer S;Hewitt A;Sokolov NA;Erlichman JS;Woodworth CD
皮肤上皮细胞在衰老过程中的尺寸增加是众所周知的。在这里，我们证明了用细胞松弛素B处理衰老细胞实质上减小了细胞大小。这种减少在小鼠模型和体外人皮肤细胞上得到了证实。通过在背部左中部皮肤上局部施用细胞松弛素B治疗六只裸鼠140天（右侧用作安慰剂治疗的对照）。结果细胞大小平均减少56±16%。在不同体外年龄的原代人皮肤上皮细胞上也观察到细胞大小的减小（从1至8代）。将从6名人类受试者的库中获得的细胞株在体外用细胞松弛素B处理12小时。我们观察到细胞大小的减少，其变得具有统计学显著性，并且对于较老传代（6-8代）的细胞达到20-40%，而对于较年轻的细胞没有观察到实质性变化。这些结果对于理解衰老过程和衰老皮肤的美容治疗可能是重要的。
The size increase of skin epithelial cells during aging is well-known. Here we demonstrate that treatment of aging cells with cytochalasin B substantially decreases cell size. This decrease was demonstrated on a mouse model and on human skin cells in vitro. Six nude mice were treated by topical application of cytochalasin B on skin of the dorsal left midsection for 140 days (the right side served as control for placebo treatment). An average decrease in cell size of 56±16% resulted. A reduction of cell size was also observed on primary human skin epithelial cells of different in vitro age (passages from 1 to 8). A cell strain obtained from a pool of 6 human subjects was treated with cytochalasin B in vitro for 12 hours. We observed a decrease in cell size that became statistically significant and reached 20–40% for cells of older passage (6–8 passages) whereas no substantial change was observed for younger cells. These results may be important for understanding the aging processes, and for cosmetic treatment of aging skin.
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影响因子：
2.2
作者：
Guz, N. V.;Gaikwad, R. M.;Sokolov, I.
通讯作者：
Sokolov, I.
影响因子：
3.5
作者：
Berdyyeva, TK;Woodworth, CD;Sokolov, I
通讯作者：
Sokolov, I
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
DOI：
10.1016/j.nano.2005.12.002
发表时间：
2006-03-01
影响因子：
5.4
作者：
Sokolov, Igor;Iyer, Swaminathan;Woodworth, Craig D.
通讯作者：
Woodworth, Craig D.
影响因子：
3.6
作者：
LEVEQUE, JL;CORCUFF, P;AGACHE, P
通讯作者：
AGACHE, P