ATP prevents Woronin bodies from sealing septal pores in unwounded cells of the fungus Zymoseptoria tritici.
ATP prevents Woronin bodies from sealing septal pores in unwounded cells of the fungus Zymoseptoria tritici.
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DOI：
10.1111/cmi.12764
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发表时间：
2017-11
影响因子：
3.4
通讯作者：
Correia A
中科院分区：
文献类型：
--
作者：
Steinberg G;Schuster M;Hacker C;Kilaru S;Correia A
丝状子囊菌的隔膜被隔膜孔穿孔，隔膜孔允许单个菌丝隔室之间的通信。损伤后，中隔孔迅速被Woronin小体（WB）堵塞，从而防止广泛的细胞质出血。WB移位到孔中的机制尚不清楚，但已表明伤口诱导的细胞质出血将WB”冲入“间隔开口。或者，隔膜相关栓系蛋白的收缩可能将WB拉入隔膜孔。在这里，我们调查WB在小麦病原菌Zymoseptoria triumphia的动态。超微结构研究表明，3.4 ± 0.2个WB位于隔膜的每一侧，直径为128.5 ± 3.6 nm的单个WB密封隔膜孔（41 ± 1.5 nm）。活细胞成像的绿色荧光ZtHex 1，一个主要的蛋白质在WB，和完整的质膜蛋白ZtSso 1证实WB易位到中隔孔。这与偶尔形成的质膜“气球”有关，挤压到死细胞中，表明质膜迅速密封受伤的隔膜孔伤口。少量的荧光ZtHex 1-增强的绿色荧光蛋白（eGFP）似乎与“气球样”质膜相关，表明细胞质ZtHex 1-eGFP被募集到延伸的质膜。令人惊讶的是，在约15%的所有病例中，WB从破裂的细胞进入中隔孔。这种易位对细胞质流表明，一个积极的机制驱动WB堵塞。事实上，用呼吸抑制剂羰基氰间氯苯腙处理未受伤和完整的细胞诱导WB移位到孔中。此外，羰基氰化物间氯苯腙处理将细胞质ZtHex 1-eGFP募集到细胞的侧质膜。因此，在三角藻中，将WB保持在隔膜孔之外是一个ATP依赖性过程。
Septa of filamentous ascomycetes are perforated by septal pores that allow communication between individual hyphal compartments. Upon injury, septal pores are plugged rapidly by Woronin bodies (WBs), thereby preventing extensive cytoplasmic bleeding. The mechanism by which WBs translocate into the pore is not known, but it has been suggested that wound‐induced cytoplasmic bleeding “flushes” WBs into the septal opening. Alternatively, contraction of septum‐associated tethering proteins may pull WBs into the septal pore. Here, we investigate WB dynamics in the wheat pathogen Zymoseptoria tritici. Ultrastructural studies showed that 3.4 ± 0.2 WBs reside on each side of a septum and that single WBs of 128.5 ± 3.6 nm in diameter seal the septal pore (41 ± 1.5 nm). Live cell imaging of green fluorescent ZtHex1, a major protein in WBs, and the integral plasma membrane protein ZtSso1 confirms WB translocation into the septal pore. This was associated with the occasional formation of a plasma membrane “balloon,” extruding into the dead cell, suggesting that the plasma membrane rapidly seals the wounded septal pore wound. Minor amounts of fluorescent ZtHex1‐enhanced green fluorescent protein (eGFP) appeared associated with the “ballooning” plasma membrane, indicating that cytoplasmic ZtHex1‐eGFP is recruited to the extending plasma membrane. Surprisingly, in ~15% of all cases, WBs moved from the ruptured cell into the septal pore. This translocation against the cytoplasmic flow suggests that an active mechanism drives WB plugging. Indeed, treatment of unwounded and intact cells with the respiration inhibitor carbonyl cyanide m‐chlorophenyl hydrazone induced WB translocation into the pores. Moreover, carbonyl cyanide m‐chlorophenyl hydrazone treatment recruited cytoplasmic ZtHex1‐eGFP to the lateral plasma membrane of the cells. Thus, keeping the WBs out of the septal pores, in Z. tritici, is an ATP‐dependent process.
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影响因子：
3.6
作者：
Beck, Julia;Echtenacher, Bernd;Ebel, Frank
通讯作者：
Ebel, Frank
影响因子：
1.5
作者：
Suzuki Y;Yura K
通讯作者：
Yura K
影响因子：
4.6
作者：
Leonhardt Y;Kakoschke SC;Wagener J;Ebel F
通讯作者：
Ebel F
DOI：
10.1073/pnas.1207467109
发表时间：
2012-09-25
影响因子：
11.1
作者：
Lai, Julian;Koh, Chuan Hock;Jedd, Gregory
通讯作者：
Jedd, Gregory
DOI：
10.1016/j.bbrc.2005.03.233
发表时间：
2005-06-17
影响因子：
3.1
作者：
Maruyama, JI;Juvvadi, PR;Kitamoto, K
通讯作者：
Kitamoto, K