In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold.
In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold.
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氧化石墨烯纳米片上羟基磷灰石纳米棒的原位合成及其在生物聚合物支架中的增强
DOI：
10.1016/j.jare.2021.03.009
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发表时间：
2022-01
影响因子：
10.7
通讯作者：
Min A
中科院分区：
文献类型：
--
作者：
Shuai C;Peng B;Feng P;Yu L;Lai R;Min A
以GO为原料，通过水热反应原位合成GO-HAP。将GO-HAP与PLLA共混，通过SLS制备生物聚合物支架。在GO上均匀修饰的HAP直径约为5 nm，长度约为60 nm。GO-HAP的加入提高了PLLA支架的抗压强度和弹性模量。PLLA/GO-HAP支架具有良好的生物活性和细胞相容性。骨组织工程中迫切需要开发具有良好力学性能和生物活性的复合骨支架。结合氧化石墨烯（GO）和羟基磷灰石（HAP）用于生物聚合物骨支架的增强已成为一种有前途的策略。然而，GO和HAP的分散仍然是一个很大的挑战。本论文通过原位合成的方法，将GO的力学性能与HAP和GO的生物活性结合起来，制备了生物高分子骨支架材料。以GO纳米片为原料，通过水热反应原位合成了GO-HAP纳米复合材料，其丰富的含氧基团作为锚位点与Ca 2+螯合，Ca 2+通过共价键吸附HPO 42-，形成均匀分散的HAP纳米棒。因此，GO-HAP纳米复合材料与生物聚合物聚-L-乳酸（PLLA）共混，用于通过选择性激光烧结（SLS）制备生物聚合物支架。GO纳米片均匀地被HAP纳米棒修饰，其长度约为60 nm，直径约为5 nm。PLLA/12%GO-HAP复合材料的抗压强度和模量分别比纯PLLA支架材料提高了53.71%和98.80%，并通过拔出、裂纹桥接、挠曲和钉扎机制进行了解释。同时，矿化实验表明PLLA/GO-HAP支架具有良好的生物活性，可诱导磷灰石层的形成。此外，细胞培养实验表明，支架具有良好的细胞相容性，可促进细胞的粘附和增殖。本研究结果显示了原位合成PLLA/GO-HAP复合支架在骨组织工程中的应用潜力。
GO was employed to in situ synthesize GO-HAP via hydrothermal reaction. GO-HAP was blended with PLLA to fabricate biopolymer scaffold via SLS. Uniformly decorated HAP on GO was about 5 nm in diameter and 60 nm in length. The adddition of GO-HAP enhanced compressive strength and modulus of PLLA scaffold. The PLLA/GO-HAP scaffold displayed good bioactivity and favorable cytocompatibility. It is urgently needed to develop composite bone scaffold with excellent mechanical properties and bioactivity in bone tissue engineering. Combining graphene oxide (GO) and hydroxyapatite (HAP) for the reinforcement of biopolymer bone scaffold has emerged as a promising strategy. However, the dispersion of GO and HAP remains to be a big challenge. In this present work, the mechanical properties of GO and the bioactivity of and HAP were combined respectively via in situ synthesis for reinforcing biopolymer bone scaffold. GO nanosheets were employed to in situ synthesize GO-HAP nanocomposite via hydrothermal reaction, in which their abundant oxygen-containing groups served as anchor sites for the chelation of Ca2+ and then Ca2+ absorbed HPO42- via electrovalent bonding to form homogeneously dispersed HAP nanorods. Thereby, the GO-HAP nanocomposite was blended with biopolymer poly-L-lactic acid (PLLA) for fabricating biopolymer scaffold by selective laser sintering (SLS). GO nanosheets were uniformly decorated with HAP nanorods, which were about 60 nm in length and 5 nm in diameter. The compressive strength and modulus of PLLA/12%GO-HAP were significantly increased by 53.71% and 98.80% compared to the pure PLLA scaffold, respectively, explained on the base of pull out, crack bridging, deflection and pinning mechanisms. Meanwhile, the mineralization experiments indicated the PLLA/GO-HAP scaffold displayed good bioactivity by inducing the formation of apatite layer. Besides, cell culturing experiments demonstrated the favorable cytocompatibility of scaffold by promoting cell adhesion and proliferation. The present findings show the potential of PLLA/GO-HAP composite scaffold via in situ synthesis in bone tissue engineering.
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影响因子：
10.9
作者：
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通讯作者：
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