Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform.
Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform.
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DOI：
10.3390/biom11020326
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发表时间：
2021-02-21
期刊：
影响因子：
5.5
通讯作者：
Ádány R
中科院分区：
文献类型：
--
作者：
Pikó P;Pál L;Szűcs S;Kósa Z;Sándor J;Ádány R
无论是在发达国家还是发展中国家，肥胖都是一个日益严重的公共卫生问题。先前的研究表明，脂类代谢的显著改变以及由此导致的脂谱的显着变化与肥胖相关并发症的发生和发展有关。为了描述肥胖引起的脂代谢变化的全谱，直接输注串联质谱分析是最有前途的方法。为了更好地了解许多脂质物种中哪些与肥胖最密切相关，我们工作的目的是使用有针对性的定量脂质组学平台Lipidyzer测量并分析从匈牙利普通人群和罗姆人样本中随机选择的正常(n=57)、超重(n=31)和肥胖(n=48)个体的血脂水平。使用主成分分析和逐步回归分析，通过增加体重指数(BMI)来识别最显著的类脂簇和种类。在18个类群中，4个关键脂类(PEP-16：0/20：3、TG 20：4_33：1、TG 22：6_36：4、TG 18：3_33：0)与BMI呈显著正相关，其余3个与BMI呈显著负相关(Hex-Cer18：1；O2/22：0、LPC 18：2、PC 18：1~18：1)。与单独的脂质物种相比，我们引入的脂质物种比率(LSR)显示出极强的相关性，至少高出9个数量级，与BMI有关。LSR可作为监测肥胖相关血浆变化和控制肥胖相关非传染性疾病治疗效果的敏感和预测指标。
Obesity is an increasing public health concern both in the developed and developing countries. Previous studies have demonstrated that considerable alterations in lipid metabolism and consequently marked changes in lipid profile are associated with the onset and progression of obesity-related complications. To characterize the full spectrum of obesity-induced changes in lipid metabolism, direct infusion tandem mass spectrometry analysis is the most promising approach. To better understand which of the many lipid species are the most strongly associated with obesity, the aim of our work was to measure and profile plasma lipids in normal (n = 57), overweight (n = 31), and obese (n = 48) individuals randomly selected from samples of Hungarian general and Roma populations by using the targeted quantitative lipidomics platform, the Lipidyzer. Principal component and stepwise regression analyses were used to identify the most significant clusters and species of lipids by increasing body mass index (BMI). From the 18 clusters identified four key lipid species (PE P-16:0/20:3, TG 20:4_33:1, TG 22:6_36:4, TG 18:3_33:0) showed a strong significant positive and three others (Hex-Cer 18:1;O2/22:0, LPC 18:2, PC 18:1_18:1) significant negative association with BMI. Compared to individual lipid species alone, the lipid species ratio (LSR) we introduced showed an extremely strong, at least 9 orders of magnitude stronger, association with BMI. The LSR can be used as a sensitive and predictive indicator to monitor obesity-related alterations in human plasma and control the effectiveness of treatment of obesity associated non-communicable diseases.
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影响因子：
2
作者：
Khan, Mostafa J.;Codreanu, Simona G.;Goyal, Sandeep;Wages, Phillip A.;Gorti, Santosh K. K.;Pearson, Mackenzie J.;Uribe, Isabel;Sherrod, Stacy D.;McLean, John A.;Porter, Ned A.;Robinson, Rena A. S.
通讯作者：
Robinson, Rena A. S.
影响因子：
4.3
作者：
Alarcon-Barrera, Juan Carlos;von Hegedus, Johannes H.;Giera, Martin
通讯作者：
Giera, Martin
影响因子：
4.8
作者：
Donovan EL;Pettine SM;Hickey MS;Hamilton KL;Miller BF
通讯作者：
Miller BF
影响因子：
4.1
作者：
Candi, Eleonora;Tesauro, Manfredi;Melino, Gerry
通讯作者：
Melino, Gerry
影响因子：
5.9
作者：
Klop B;Elte JW;Cabezas MC
通讯作者：
Cabezas MC