Lysyl oxidase-mediated collagen crosslinks may be assessed as markers of functional properties of tendon tissue formation.
Lysyl oxidase-mediated collagen crosslinks may be assessed as markers of functional properties of tendon tissue formation.
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DOI：
10.1016/j.actbio.2013.11.024
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发表时间：
2014-03
影响因子：
9.7
通讯作者：
Kuo, Catherine K.
中科院分区：
文献类型：
--
作者：
Marturano, Joseph E.;Xylas, Joanna F.;Sridharan, Gautham V.;Georgakoudi, Irene;Kuo, Catherine K.
工程组织的机械性能研究通常是基于基因和蛋白质的特性，而不是机械测试。然而，我们最近证明，在胚胎组织发育过程中，机械性能与基质含量和组织并不一致。在此基础上，在自然或工程组织形成过程中，应独立评估机械性能。不幸的是，机械测试是破坏性的，因此评估这些性能的替代方法是可取的。在这项研究中，我们研究了赖氨酰氧化酶(LOX)介导的交联物作为胚胎肌腱形成过程中力学特性的标志物以及非破坏性检测它们的可能性。我们用串联质谱仪(LC-MS/MS)定量测定了鸡胚胎肌腱中羟基吡啶(HP)和赖氨酰吡啶(LP)交联物密度随发育阶段的变化。此外，我们评估了一种利用HP和LP的自然荧光的多光子成像方法。使用这两种技术，我们量化了正常肌腱和LOX抑制肌腱中的交联物密度，并将测量结果与力学性能进行了关联。高蛋白和低蛋白的交联度随发育阶段的不同而变化，高蛋白与干物质量比与弹性模数高度相关，即使酶促交联剂的形成受到抑制。多光子光学成像证实了LC-MS/MS数据，发现LOX抑制导致交联物密度显著降低。综上所述，交联剂密度可能是有用的组织机械性能的标记，可以通过成像进行非破坏性或非侵入性的评估。这些结果可能具有重大的科学和临床意义，使连续和长期监测胶原交联组织或工程结构的机械性能成为可能。
Mechanical property elaboration of engineered tissues is often assumed on the basis of gene and protein characterizations, rather than mechanical testing. However, we recently demonstrated mechanical properties are not consistently correlated with matrix content and organization during embryonic tissue development. Based on this, mechanical properties should be assessed independently during natural or engineered tissue formation. Unfortunately, mechanical testing is destructive, and thus alternative means of assessing these properties are desirable. In this study, we examined lysyl oxidase (LOX)-mediated crosslinks as markers for mechanical properties during embryonic tendon formation and the potential to detect them non-destructively. We used tandem mass spectrometry (LC-MS/MS) to quantify changes in hydroxylysyl pyridinoline (HP) and lysyl pyridinoline (LP) crosslink density in embryonic chick tendon as a function of developmental stage. In addition, we assessed a multiphoton imaging approach that exploits the natural fluorescence of HP and LP. With both techniques, we quantified crosslink density in normal and LOX-inhibited tendons, and correlated measurements with mechanical properties. HP and LP crosslink density varied as a function of developmental stage, with HP-to-dry mass ratio correlating highly to elastic modulus, even when enzymatic crosslink formation was inhibited. Multiphoton optical imaging corroborated LC-MS/MS data, identifying significant reductions in crosslink density from LOX inhibition. Taken together, crosslink density may be useful as a marker of tissue mechanical properties that could be assessed with imaging non-destructively and perhaps non-invasively. These outcomes could have significant scientific and clinical implications, enabling continuous and long-term monitoring of mechanical properties of collagen-crosslinked tissues or engineered constructs.
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影响因子：
2.8
作者：
FRANK, C;MCDONALD, D;SHRIVE, N
通讯作者：
SHRIVE, N
影响因子：
4.8
作者：
Eyre, David R.;Weis, Mary Ann;Wu, Jiann-Jiu
通讯作者：
Wu, Jiann-Jiu
影响因子：
4
作者：
Kagan, HM;Li, WD
通讯作者：
Li, WD
DOI：
10.1016/s0731-7085(03)00359-5
发表时间：
2003-12-04
影响因子：
3.4
作者：
Kindt, E;Gueneva-Boucheva, K;Hallak, H
通讯作者：
Hallak, H
影响因子：
3.7
作者：
Farlay D;Duclos ME;Gineyts E;Bertholon C;Viguet-Carrin S;Nallala J;Sockalingum GD;Bertrand D;Roger T;Hartmann DJ;Chapurlat R;Boivin G
通讯作者：
Boivin G