Mechanical stress analysis of microfluidic environments designed for isolated biological cell investigations.
Mechanical stress analysis of microfluidic environments designed for isolated biological cell investigations.
复制标题
DOI：
10.1115/1.4000121
复制
发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Tretheway DC
中科院分区：
文献类型：
--
作者：
Kohles SS;Nève N;Zimmerman JD;Tretheway DC
在细胞水平上评估生物力学的技术的进步已经导致了机械转导的发现和作为疾病生物标志物的细胞力学的研究。随着具有微米分辨率粒子图像测速仪的集成光学镊子的最新发展，可获得向悬浮的单细胞施加受控的多轴应力的机会（Nève，N.，林伍德，J.K.，齐默尔曼，J.，Kohles，S.美国，和Tretheway，D. C.的方法，2008年，“µPIVOT：用于微环境研究的集成粒子图像测速和光镊仪器”，Meas。Sci.技术人员：19（9），pp. 095403）。一个应力分析被施加到实验和理论的流速梯度的悬浮细胞大小的聚苯乙烯微球展示相关的几何形状的非粘附的球形细胞，观察成骨细胞，软骨细胞和成纤维细胞。三个流动条件进行了评估：一个均匀的流场，通过移动的流体样品与自动平移台，重力驱动的流动通过直的微通道，和重力驱动的流动通过微通道交叉路口。分析表明，悬浮细胞上的流体诱导应力（流体动力学剪切应力、法向应力和主应力在0.02-0.04 Pa范围内）通常比均匀和直微通道流动的粘附单细胞研究（0.5-1.0 Pa）低至少一个数量级。此外，流体静压力（1-100 Pa）超过流体动力应力。然而，在交叉结配置中，在没有物理接触的影响并且具有最小激光捕获功率的情况下，可能存在数量级更大的流体动力学应力。
Advancements in technologies for assessing biomechanics at the cellular level have led to discoveries in mechanotransduction and the investigation of cell mechanics as a biomarker for disease. With the recent development of an integrated optical tweezer with micron resolution particle image velocimetry, the opportunity to apply controlled multiaxial stresses to suspended single cells is available (Nève, N., Lingwood, J. K., Zimmerman, J., Kohles, S. S., and Tretheway, D. C., 2008, “The µPIVOT: An Integrated Particle Image Velocimetry and Optical Tweezers Instrument for Microenvironment Investigations,” Meas. Sci. Technol., 19(9), pp. 095403). A stress analysis was applied to experimental and theoretical flow velocity gradients of suspended cell-sized polystyrene microspheres demonstrating the relevant geometry of nonadhered spherical cells, as observed for osteoblasts, chondrocytes, and fibroblasts. Three flow conditions were assessed: a uniform flow field generated by moving the fluid sample with an automated translation stage, a gravity driven flow through a straight microchannel, and a gravity driven flow through a microchannel cross junction. The analysis showed that fluid-induced stresses on suspended cells (hydrodynamic shear, normal, and principal stresses in the range of 0.02–0.04 Pa) are generally at least an order of magnitude lower than adhered single cell studies for uniform and straight microchannel flows (0.5–1.0 Pa). In addition, hydrostatic pressures dominate (1–100 Pa) over hydrodynamic stresses. However, in a cross junction configuration, orders of magnitude larger hydrodynamic stresses are possible without the influence of physical contact and with minimal laser trapping power.
登录
查看更多内容
影响因子：
3.4
作者：
Neuman, KC;Chadd, EH;Block, SM
通讯作者：
Block, SM
影响因子：
3.5
作者：
Walker, LM;Holm, Å;El Haj, AJ
通讯作者：
El Haj, AJ
影响因子：
2
作者：
Meinhart, CD;Wereley, ST;Santiago, JG
通讯作者：
Santiago, JG
DOI：
10.1115/1.2165689
发表时间：
2006-04-01
影响因子：
1.7
作者：
Leyton-Mange, J;Yang, S;Dong, C
通讯作者：
Dong, C
影响因子：
3.4
作者：
Guck, J;Ananthakrishnan, R;Käs, J
通讯作者：
Käs, J