A Synthetic Formula Amino Acid Diet Leads to Microbiome Dysbiosis, Reduced Colon Length, Inflammation, and Altered Locomotor Activity in C57BL/6J Mice.
A Synthetic Formula Amino Acid Diet Leads to Microbiome Dysbiosis, Reduced Colon Length, Inflammation, and Altered Locomotor Activity in C57BL/6J Mice.
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DOI：
10.3390/microorganisms11112694
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发表时间：
2023-11-03
期刊：
影响因子：
4.5
通讯作者：
Allen MS
中科院分区：
文献类型：
--
作者：
Mancilla VJ;Braden-Kuhle PN;Brice KN;Mann AE;Williams MT;Zhang Y;Chumley MJ;Barber RC;White SN;Boehm GW;Allen MS
合成的游离氨基酸饮食，类似于(苯丙酮尿症)PKU患者的补充剂，对肠道微生物区系和整体健康的影响尚不清楚。在目前的多学科研究中，我们检查了合成来源的低纤维氨基酸饮食对行为、认知、肠道微生物群组成和炎症标志物的影响。20只雄性C57BL/6J小鼠在出生后第21天随机分为标准饲料组和合成饲料组(n=10)，连续饲养13周。对粪便样本中16S rRNA基因的测序显示，在合成饲料组中，细菌多样性降低，与疾病相关的细菌(如普雷沃特氏菌)数量增加，与发酵途径相关的肠道微生物群下降。此外，在食用合成饮食的小鼠中，短链脂肪酸水平降低，结肠缩短。最后，我们检测了血清、海马体和结肠中的肿瘤坏死因子-α、IL-6和IL-10，发现合成饲料显著增加了海马区IL-6的产生。这些结果表明了多学科方法对未来饮食和微生物组研究的重要性，因为饮食不仅影响肠道微生物组组成，而且潜在地影响全身健康。
The effects of synthetic, free-amino acid diets, similar to those prescribed as supplements for (phenylketonuria) PKU patients, on gut microbiota and overall health are not well understood. In the current, multidisciplinary study, we examined the effects of a synthetically-derived, low-fiber, amino acid diet on behavior, cognition, gut microbiome composition, and inflammatory markers. A cohort of 20 male C57BL/6J mice were randomly assigned to either a standard or synthetic diet (n = 10) at post-natal day 21 and maintained for 13 weeks. Sequencing of the 16S rRNA gene from fecal samples revealed decreased bacterial diversity, increased abundance of bacteria associated with disease, such as Prevotella, and a downward shift in gut microbiota associated with fermentation pathways in the synthetic diet group. Furthermore, there were decreased levels of short chain fatty acids and shortening of the colon in mice consuming the synthetic diet. Finally, we measured TNF-α, IL-6, and IL-10 in serum, the hippocampus, and colon, and found that the synthetic diet significantly increased IL-6 production in the hippocampus. These results demonstrate the importance of a multidisciplinary approach to future diet and microbiome studies, as diet not only impacts the gut microbiome composition but potentially systemic health as well.
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影响因子：
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