Development of quantitative immunochromatographic assay for rapid and sensitive detection of carbohydrate antigen 19-9 (CA 19-9) in human plasma.
Development of quantitative immunochromatographic assay for rapid and sensitive detection of carbohydrate antigen 19-9 (CA 19-9) in human plasma.
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DOI：
10.1016/j.jpba.2017.09.004
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发表时间：
2017-11-30
影响因子：
3.4
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Baryeh K;Takalkar S;Lund M;Liu G
建立了一种基于金纳米颗粒（GNP）的侧流试纸条生物传感器（LFSB）和便携式试纸条阅读器的免疫层析定量分析方法（QIA），用于人血浆中糖抗原19-9（CA 19-9）的快速、灵敏定量。CA 19-9是与癌症（如胰腺癌和结肠直肠癌）和各种非癌性疾病相关的生物标志物。其原理是利用纳米金（GNP）标记的检测抗体、抗CA 19-9捕获抗体和CA 19- 9之间的免疫反应，将GNP捕获在LFSB的检测区。GNP在测试区上的积累给出红线，用便携式条读取器读取其强度以定量CA 19-9的浓度。优化了包括膜类型、抗体浓度、GNP-抗CA 19-9缀合物的量和运行缓冲液的组分在内的测定参数，以获得测定的最佳灵敏度和重现性。测定的检测限为5 U mL−1（S/N=3），线性范围为5 U mL−1至100 U mL−1。应用建立的定量免疫层析法（QIA）成功地测定了健康人和胰腺癌患者血浆中CA 19-9的浓度，其结果与酶联免疫吸附试验（ELISA）的结果一致。所开发的测定显示出巨大的前景，临床应用和生物医学诊断，特别是在有限的资源设置。建立了一种基于金纳米颗粒的侧流试纸条生物传感器和便携式试纸条阅读器的免疫层析定量分析方法，用于快速、灵敏地定量健康人和胰腺癌患者血浆中的碳水化合物抗原19-9（CA 19-9）。
A quantitative immunochromatographic assay (QIA) was developed by using gold nanoparticle (GNP)-based lateral flow strip biosensor (LFSB) and a portable strip reader for rapid and sensitive quantitation of Carbohydrate Antigen 19-9 (CA 19-9) in human plasma. CA 19-9 is a biomarker that has been associated with cancers (such as pancreatic and colorectal cancers) and various non-cancerous diseases. The principle is based on sandwich-type immunoreactions between gold nanoparticle (GNP)-labelled detection antibody, anti-CA 19-9 capture antibody and CA 19-9to capture the GNPs on the test zone of LFSB. The accumulation of GNPs on the test zone gave a red line whose intensity was read with a portable strip reader to quantify the concentration of CA 19-9. Assay parameters including the membrane type, antibody concentration, amount of GNP-anti-CA 19-9 conjugates and the components of the running buffer were optimized to obtain the best sensitivity and reproducibility of the assay. The detection limit of the assay was determined to be 5 U mL−1 (S/N=3) with a linear range of 5 U mL−1 to 100 U mL−1. CA 19-9 concentrations in healthy human and pancreatic cancer patient plasma samples were successfully evaluated using the developed quantitative immunochromatographic assay (QIA), and the results were in accordance with that obtained with enzyme linked immunosorbent assay (ELISA). The developed assay shows great promise for clinical application and biomedical diagnosis, particularly in limited resource settings. A quantitative immunochromatographic assay was developed by using gold nanoparticle-based lateral flow strip biosensor and a portable strip reader for rapid and sensitive quantitation of Carbohydrate Antigen 19-9 (CA 19-9) in healthy human and pancreatic cancer patient plasma.
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影响因子：
8.4
作者：
Chung, J. W.;Bernhardt, R.;Pyun, J. C.
通讯作者：
Pyun, J. C.
影响因子：
7.4
作者：
GRABAR, KC;FREEMAN, RG;NATAN, MJ
通讯作者：
NATAN, MJ
影响因子：
56.9
作者：
KOPROWSKI, H;HERLYN, M;SEARS, HF
通讯作者：
SEARS, HF
基于改进的单基因信号微分相位一致性的边缘检测方法
影响因子：
2.5
作者：
Yang, Yan;Kou, Kit Ian;Zou, Cuiming
通讯作者：
Zou, Cuiming
DOI：
10.1007/bf01542654
发表时间：
1979-01-01
期刊：
SOMATIC CELL GENETICS
影响因子：
--
作者：
KOPROWSKI, H;STEPLEWSKI, Z;FUHRER, P
通讯作者：
FUHRER, P