DNA-protein crosslinking by heavy metals in Novikoff hepatoma.
DNA-protein crosslinking by heavy metals in Novikoff hepatoma.
复制标题
Novikoff 肝癌中重金属导致的 DNA-蛋白质交联。
DOI：
10.1016/0003-9861(86)90345-0
复制
发表时间：
1986
影响因子：
3.9
通讯作者：
Hnilica,LS
中科院分区：
文献类型：
--
作者：
Wedrychowski,A;Schmidt,WN;Hnilica,LS
在体外暴露于铬 VI、III 和 II、镍 II、镉 II 盐以及 CoCl2、As2O3 和 AlK(SO4)2 的活完整 Novikoff 腹水肝癌细胞中研究了蛋白质与 DNA 的交联。通过高速离心溶解在缓冲的 4% 十二烷基硫酸钠中的细胞来分离 DNA-蛋白质复合物，并通过聚丙烯酰胺凝胶电泳进行测定。六价铬化合物非常有效地形成DNA-蛋白质复合物。三价、难溶性亚铬酸铜几乎与六价铬一样有效的交联剂，这可能是因为吞噬作用促进了其进入细胞。更碱性的二价形式几乎不产生任何交联。大多数交联蛋白对于所使用的所有铬盐来说是共有的。镍盐形成 DNA-蛋白质交联的效率较低。大多数由这种金属交联的蛋白质都具有 94,000 至 200,000 的高分子量。几种不同的镍 (II) 盐的交联蛋白质模式之间几乎没有质量差异。用镉盐孵育的细胞也获得了类似的结果。大多数镉交联的蛋白质具有高分子量，并且与镍（II）交联的蛋白质相似。当 Novikoff 肝癌细胞暴露于 CoCl2、As2O3 或 AlK(SO4)2 时，也观察到相对较弱但显着的交联。
Crosslinking of proteins to DNA was studied in live intact Novikoff ascites hepatoma cells exposedin vitroto salts of chromium VI, III, and II, nickel II, cadmium II, and to CoCl2, As2O3, and AlK(SO4)2. DNA-protein complexes were separated by high-speed centrifugation of cells solubilized in buffered 4% sodium dodecyl sulfate and assayed by polyacrylamide gel electrophoresis. Hexavalent chromium compounds formed DNA-protein complexes very efficiently. The trivalent, poorly soluble, cupric chromite was nearly as efficient crosslinker as hexavalent Cr, perhaps because phagocytosis facilitated its entry into the cells. The more basic divalent form produced hardly any crosslinks. Most of the crosslinked proteins were common to all of the chromium salts employed. Nickel salts formed DNA-protein crosslinks less efficiently. Most proteins crosslinked by this metal had a high molecular weight ranging from 94,000 to 200,000. There was little qualitative difference between the crosslinked protein patterns for several various nickel (II) salts. Similar results were obtained for cells incubated with cadmium salts. Most of the proteins crosslinked by cadmium had high molecular weights and were similar to those crosslinked by nickel (II). Relatively weak, but significant, Crosslinking was also observed when the Novikoff hepatoma cells were exposed to CoCl2, As2O3, or AlK(SO4)2.
登录
查看更多内容
金属对诱变和 DNA 修复的影响。
影响因子：
10.4
作者：
T. Rossman
通讯作者：
T. Rossman
大鼠肝微粒体对致癌物质铬酸盐的代谢。
DOI：
10.1016/0006-291x(78)90931-2
发表时间：
1978
影响因子：
3.1
作者：
J. Gruber;K. Jennette
通讯作者：
K. Jennette
六价和三价铬在体外对哺乳动物细胞的细胞毒性作用。
影响因子：
8.8
作者：
Levis, A G;Bianchi, V;Tamino, G;Pegoraro, B
通讯作者：
Pegoraro, B
修复鸡胚肝细胞中铬酸盐诱导的 DNA 损伤。
影响因子：
4.7
作者：
Cupo,DY;Wetterhahn,KE
通讯作者：
Wetterhahn,KE
致癌物质铬酸盐会诱导大鼠肝脏和肾脏中的 DNA 交联。
DOI：
10.1016/s0021-9258(19)69495-0
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Tsapakos;T. Hampton;K. Jennette
通讯作者：
K. Jennette