Transgenic microRNA-14 rice shows high resistance to rice stem borer.
Transgenic microRNA-14 rice shows high resistance to rice stem borer.
复制标题
转基因 microRNA-14 水稻表现出对二化螟的高抗性
DOI：
10.1111/pbi.12990
复制
发表时间：
2019-03
影响因子：
13.8
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
He K;Xiao H;Sun Y;Ding S;Situ G;Li F
二化螟是一种每年造成巨大经济损失的害虫。控制工作严重依赖化学杀虫剂，这导致了杀虫剂抗药性、环境污染和食品安全等严重问题。因此，开发替代的害虫防治方法是一项重要的任务。在这里，我们在RSB中发现了一个昆虫特异性的microRNA，miR-14，它被预测为针对蜕皮激素信号网络中的Spook(Spo)和蜕皮激素受体(ECR)。HEK293T细胞体外双荧光素酶检测证实CSU-miR-14与CsSpo和CsEcR相互作用。CSU-miR-14在幼虫各龄期末期均有较高水平的表达，其表达与两个靶基因的表达呈负相关。在5龄第3天过表达CSU-miR-14会导致RSB个体高死亡率和发育缺陷。我们获得了35个水稻转化子来表达miR-14，发现三个品系都有一个拷贝，含有高度丰富的miR-14成熟转录本。T0代和T1代转基因miR-14水稻的饲养鉴定表明，至少有一个品系(C#24)表现出对赤霉病的高抗。这些结果表明，以miRNAs为靶标的方法具有改进害虫防治方法的潜力。此外，使用昆虫特有的miRNAs而不是编码蛋白质的基因来控制害虫，可能会证明对非昆虫物种是无害的，因此值得进一步探索。
Rice stem borer (RSB, Chilo suppressalis) is an insect pest that causes huge economic losses every year. Control efforts rely heavily on chemical insecticides, which leads to serious problems such as insecticide resistance, environment pollution, and food safety issues. Therefore, developing alternative pest control methods is an important task. Here, we identified an insect‐specific microRNA, miR‐14, in RSB, which was predicted to target Spook (Spo) and Ecdysone receptor (EcR) in the ecdysone signalling network. In‐vitro dual luciferase assays using HEK293T cells confirmed the interactions of Csu‐miR‐14 with CsSpo and with CsEcR. Csu‐miR‐14 exhibited high levels of expression at the end of each larval instar stage, and its expression was negatively correlated with the expression of its two target genes. Overexpression of Csu‐miR‐14 at the third day of the fifth instar stage led to high mortality and developmental defects in RSB individuals. We produced 35 rice transformants to express miR‐14 and found that three lines had a single copy with highly abundant miR‐14 mature transcripts. Feeding bioassays using both T0 and T1 generations of transgenic miR‐14 rice indicated that at least one line (C#24) showed high resistance to RSB. These results indicated that the approach of miRNAs as targets has potential for improving pest control methods. Moreover, using insect‐specific miRNAs rather than protein‐encoding genes for pest control may prove benign to non‐insect species, and thus is worthy of further exploration.
登录
查看更多内容
影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
白背飞虱 (Sogatellafurcifera) 中 microRNA 的鉴定和表征。
影响因子：
4
作者：
Chang, Zhao-Xia;Tang, Nan;Wu, Qing-Fa
通讯作者：
Wu, Qing-Fa
影响因子：
14.9
作者：
Kalvari I;Argasinska J;Quinones-Olvera N;Nawrocki EP;Rivas E;Eddy SR;Bateman A;Finn RD;Petrov AI
通讯作者：
Petrov AI
影响因子：
3
作者：
Alfonso-Rubí, J;Ortego, F;Díaz, I
通讯作者：
Díaz, I
影响因子：
2.2
作者：
Huang Shuijin;Chen Qiong;Qin Houguo
通讯作者：
Qin Houguo