Magnetic nanoparticles in primary neural cell cultures are mainly taken up by microglia.
Magnetic nanoparticles in primary neural cell cultures are mainly taken up by microglia.
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DOI：
10.1186/1471-2202-13-32
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发表时间：
2012-03-22
期刊：
影响因子：
2.4
通讯作者：
Keilhoff G
中科院分区：
文献类型：
--
作者：
Pinkernelle J;Calatayud P;Goya GF;Fansa H;Keilhoff G
磁性纳米粒子（MNP）在生命科学中有着广泛的应用。在神经科学中的应用是感兴趣的焦点之一。不幸的是，并不是所有的团体都有机会获得纳米粒子或有可能开发和生产它们的应用。因此，他们必须专注于商业上可获得的颗粒。关于原代细胞中纳米颗粒的摄取知之甚少。以前的研究主要报道细胞系中的细胞摄取。在这里，我们提出了一个系统的研究磁性纳米粒子（MNP）的神经系统的原代细胞的摄取。我们用共聚焦显微镜和电子显微镜评估了不同细胞类型的内化。分析证实了细胞中MNP的摄取，可能具有内吞机制。此外，我们比较了在PC 12细胞，大鼠嗜铬细胞瘤细胞系，这是经常被用作神经元细胞模型，与原代神经元细胞的摄取。结果发现，装载有MNP的PC 12细胞的百分比显著高于神经元。在原代混合神经元/神经胶质细胞培养物中的摄取研究显示，小胶质细胞对MNP的摄取占主导地位，并且其数量增加。掺入MNP的星形胶质细胞和少突胶质细胞的数量较低且稳定。原代混合雪旺细胞/成纤维细胞培养物显示两种细胞类型的MNP摄取相似，但MNP孵育后雪旺细胞数量减少。脊髓切片和周围神经移植物的器官型共培养类似于分散的原代细胞培养的结果。商业MNP在分散和器官型培养系统中使用活化的小胶质细胞吞噬作用。可以假设体内应用也会诱导免疫系统反应性。正因为如此，它们在体内神经科学实施中的有用性可能受到质疑。未来的研究将需要通过使用细胞特异性靶向策略来克服这个问题。此外，我们发现PC 12细胞比原代神经元摄取更多的MNP。这种差异表明，PC 12细胞不是用于纳米颗粒的天然神经元摄取的合适模型，并且限定了先前在PC 12细胞中的结果。
Magnetic nanoparticles (MNPs) offer a large range of applications in life sciences. Applications in neurosciences are one focus of interest. Unfortunately, not all groups have access to nanoparticles or the possibility to develop and produce them for their applications. Hence, they have to focus on commercially available particles. Little is known about the uptake of nanoparticles in primary cells. Previously studies mostly reported cellular uptake in cell lines. Here we present a systematic study on the uptake of magnetic nanoparticles (MNPs) by primary cells of the nervous system. We assessed the internalization in different cell types with confocal and electron microscopy. The analysis confirmed the uptake of MNPs in the cells, probably with endocytotic mechanisms. Furthermore, we compared the uptake in PC12 cells, a rat pheochromocytoma cell line, which is often used as a neuronal cell model, with primary neuronal cells. It was found that the percentage of PC12 cells loaded with MNPs was significantly higher than for neurons. Uptake studies in primary mixed neuronal/glial cultures revealed predominant uptake of MNPs by microglia and an increase in their number. The number of astroglia and oligodendroglia which incorporated MNPs was lower and stable. Primary mixed Schwann cell/fibroblast cultures showed similar MNP uptake of both cell types, but the Schwann cell number decreased after MNP incubation. Organotypic co-cultures of spinal cord slices and peripheral nerve grafts resembled the results of the dispersed primary cell cultures. The commercial MNPs used activated microglial phagocytosis in both disperse and organotypic culture systems. It can be assumed that in vivo application would induce immune system reactivity, too. Because of this, their usefulness for in vivo neuroscientific implementations can be questioned. Future studies will need to overcome this issue with the use of cell-specific targeting strategies. Additionally, we found that PC12 cells took up significantly more MNPs than primary neurons. This difference indicates that PC12 cells are not a suitable model for natural neuronal uptake of nanoparticles and qualify previous results in PC12 cells.
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影响因子：
17.1
作者：
Jenkins, Stuart I.;Pickard, Mark R.;Chari, Divya M.
通讯作者：
Chari, Divya M.
影响因子：
--
作者：
Luciani, Nathalie;Gazeau, Florence;Wilhelm, Claire
通讯作者：
Wilhelm, Claire
DOI：
10.1023/b:jmsm.0000021126.32934.20
发表时间：
2004-04-01
影响因子：
3.7
作者：
Gupta, AK;Curtis, ASG
通讯作者：
Curtis, ASG
DOI：
10.1073/pnas.85.16.6167
发表时间：
1988-08-01
影响因子：
11.1
作者：
ALOISI, F;AGRESTI, C;LEVI, G
通讯作者：
LEVI, G
影响因子：
3.5
作者：
Li, Wei;Chen, Chunying;Zhang, Yingge
通讯作者：
Zhang, Yingge