Time course of human skeletal muscle nitrate and nitrite concentration changes following dietary nitrate ingestion.
Time course of human skeletal muscle nitrate and nitrite concentration changes following dietary nitrate ingestion.
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DOI：
10.1016/j.niox.2022.01.003
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发表时间：
2022-04-01
期刊：
影响因子：
--
通讯作者：
Jones AM
中科院分区：
文献类型：
--
作者：
Kadach S;Piknova B;Black MI;Park JW;Wylie LJ;Stoyanov Z;Thomas SM;McMahon NF;Vanhatalo A;Schechter AN;Jones AM
饮食中的硝酸盐（NO3−）摄入对健康和运动表现有益。最近，基于动物和有限的人类研究，骨骼肌NO3−储库被认为在全身一氧化氮（NO）稳态中很重要。本研究的目的是确定摄入膳食NO3−后人体骨骼肌NO3−浓度（[NO3−]）变化的时间过程。16名参与者被分配到实验组（NIT：n = 11）或安慰剂组（PLA：n = 5），其中实验组服用约1300 mg（12.8 mmol）硝酸钾（KNO 3），安慰剂组服用氯化钾（KCl）。在NIT或PLA摄入前不久以及在随后的24小时内间隔收集生物样品（肌肉（股外侧肌）、血液、唾液和尿液）。基线时，NIT和PLA之间的肌肉[NO3-]和[NO2-]无差异（P > 0.05）。在PLA中，肌肉[NO3-]或[NO2-]随时间推移没有变化。在NIT中，肌肉[NO3−]在0.5 h显著高于基线（54 ± 29 nmol/g），在3 h达到峰值（181 ± 128 nmol/g），从9 h开始与基线无差异（P > 0.05）。肌肉[NO2−]随时间推移没有显著变化。在摄入一团膳食NO3 −后，骨骼肌[NO3−]迅速增加，在约3小时达到峰值，随后下降至基线值。摄入膳食NO3-后，人类m.股外侧肌[NO3−]的药代动力学曲线与血浆[NO3−]相比略有延迟。
Dietary nitrate (NO3−) ingestion can be beneficial for health and exercise performance. Recently, based on animal and limited human studies, a skeletal muscle NO3− reservoir has been suggested to be important in whole body nitric oxide (NO) homeostasis. The purpose of this study was to determine the time course of changes in human skeletal muscle NO3− concentration ([NO3− ) following the ingestion of dietary NO3−. Sixteen participants were allocated to either an experimental group (NIT: n = 11) which consumed a bolus of ~1300 mg (12.8 mmol) potassium nitrate (KNO3), or a placebo group (PLA: n = 5) which consumed a bolus of potassium chloride (KCl). Biological samples (muscle (vastus lateralis), blood, saliva and urine) were collected shortly before NIT or PLA ingestion and at intervals over the course of the subsequent 24 h. At baseline, no differences were observed for muscle [NO3−] and [NO2−] between NIT and PLA (P > 0.05). In PLA, there were no changes in muscle [NO3−] or [NO2−] over time. In NIT, muscle [NO3−] was significantly elevated above baseline (54 ± 29 nmol/g) at 0.5 h, reached a peak at 3 h (181 ± 128 nmol/g), and was not different to baseline from 9 h onwards (P > 0.05). Muscle [NO2−] did not change significantly over time. Following ingestion of a bolus of dietary NO3− skeletal muscle [NO3−] increases rapidly, reaches a peak at ~3 h and subsequently declines towards baseline values. Following dietary NO3− ingestion, human m. vastus lateralis [NO3−] expressed a slightly delayed pharmacokinetic profile compared to plasma [NO3−].
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影响因子：
5.5
作者：
Ferguson, Scott K.;Hirai, Daniel M.;Poole, David C.
通讯作者：
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影响因子：
8.3
作者：
Kapil, Vikas;Milsom, Alexandra B.;Ahluwalia, Amrita
通讯作者：
Ahluwalia, Amrita
影响因子：
7.4
作者：
Burleigh, Mia C.;Liddle, Luke;Easton, Chris
通讯作者：
Easton, Chris
影响因子：
82.9
作者：
Cosby, K;Partovi, KS;Gladwin, MT
通讯作者：
Gladwin, MT
DOI：
10.1016/j.niox.2021.03.003
发表时间：
2021-05-01
期刊：
Nitric oxide : biology and chemistry
影响因子：
--
作者：
Park JW;Thomas SM;Schechter AN;Piknova B
通讯作者：
Piknova B