Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression.
Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression.
复制标题
DOI：
10.1038/s41586-021-04062-5
复制
发表时间：
2021-11
期刊：
影响因子：
64.8
通讯作者：
Dean C
中科院分区：
文献类型：
--
作者：
Zhu P;Lister C;Dean C
植物利用季节性的温度线索来确定过渡到繁殖的时间。在拟南芥中，冬季寒冷的表观遗传沉默的花抑制基因座FLOWERYLOCUS C（FLC）通过多孢抑制复合物2（PRC2）。这种春化过程使开花与春天保持一致。沉默的先决条件是FLC的转录下调，但这是如何发生在秋季波动的温度制度是未知的。转录抑制与组蛋白H3在K36处的三甲基化（H3K36me3）和H3在K4处的三甲基化（H3K4me3）的局部水平降低相关，其在FLC的FRIGIDA（FRI）依赖性激活期间沉积。在这里，我们表明，冷迅速促进FRI核凝聚物的形成，不与一个活跃的FLC基因座共定位。这与FLC启动子处的FRI占据率降低和FLC阻遏相关。温暖的温度峰值逆转了这一过程，缓冲了FLC关闭，以防止过早开花。冷凝物在寒冷中的积累受到特定的共转录调节因子和反义RNA COOLAIR的特定同种型的冷诱导的影响。我们的工作描述了动态分区的转录激活剂赋予可塑性，以响应自然温度波动，从而使植物能够有效地监测季节性进展。在拟南芥中，响应于冷的花阻遏物FLC的下调通过FLC激活剂FRIGIDA被隔离到远离FLC启动子的生物分子缩合物中的机制发生。
Plants use seasonal temperature cues to time the transition to reproduction. In Arabidopsis thaliana, winter cold epigenetically silences the floral repressor locus FLOWERING LOCUS C (FLC) through POLYCOMB REPRESSIVE COMPLEX 2 (PRC2). This vernalization process aligns flowering with spring. A prerequisite for silencing is transcriptional downregulation of FLC, but how this occurs in the fluctuating temperature regimes of autumn is unknown. Transcriptional repression correlates with decreased local levels of histone H3 trimethylation at K36 (H3K36me3) and H3 trimethylation at K4 (H3K4me3), which are deposited during FRIGIDA (FRI)-dependent activation of FLC. Here we show that cold rapidly promotes the formation of FRI nuclear condensates that do not colocalize with an active FLC locus. This correlates with reduced FRI occupancy at the FLC promoter and FLC repression. Warm temperature spikes reverse this process, buffering FLC shutdown to prevent premature flowering. The accumulation of condensates in the cold is affected by specific co-transcriptional regulators and cold induction of a specific isoform of the antisense RNA COOLAIR. Our work describes the dynamic partitioning of a transcriptional activator conferring plasticity in response to natural temperature fluctuations, thus enabling plants to effectively monitor seasonal progression. In Arabidopsis thaliana, downregulation of the floral repressor FLC in response to cold occurs through a mechanism in which the FLC activator FRIGIDA is sequestered into biomolecular condensates away from the FLC promoter.
登录
查看更多内容
影响因子：
9.3
作者：
Ietswaart R;Rosa S;Wu Z;Dean C;Howard M
通讯作者：
Howard M
DOI：
10.1073/pnas.1419030111
发表时间：
2014-11-11
影响因子：
11.1
作者：
Csorba, Tibor;Questa, Julia I.;Dean, Caroline
通讯作者：
Dean, Caroline
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
Dean, C
影响因子：
18
作者：
Li, Zicong;Jiang, Danhua;He, Yuehui
通讯作者：
He, Yuehui
影响因子：
10.5
作者：
He, YH;Doyle, MR;Amasino, RM
通讯作者：
Amasino, RM