Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes.
Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes.
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DOI：
10.1371/journal.pntd.0003566
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发表时间：
2015-03
影响因子：
3.8
通讯作者：
Serre D
中科院分区：
文献类型：
--
作者：
Chan ER;Barnwell JW;Zimmerman PA;Serre D
间日疟原虫的生物学已经从成功适应人类感染非人类灵长类动物的能力中获得了重要的见解。在猴子体内培养的间日疟原虫毒株可作为寄生虫的再生来源，用于体外和离体实验研究和功能分析，或用于体内研究复发特征、蚊子种类相容性、药物敏感性谱或对潜在候选疫苗的免疫反应。尽管这些研究很重要，但对于间日疟原虫对不同宿主物种的适应如何影响其基因组，我们知之甚少。此外，尚不清楚这些适应猴子的菌株是由单克隆寄生虫种群组成，还是保留了在野外分离株中常见的多克隆复杂性。在这里，我们比较了适应新世界猴子的7株间日疟原虫菌株与直接在野外收集的6株人类临床分离株的基因组序列。我们发现间日疟原虫对猴子宿主的适应及其随后的繁殖并没有导致其基因组序列的显著改变，这些适应猴子的菌株概括了野外分离株的基因组多样性。我们的分析还表明，这些菌株并不总是遗传同质的，应该谨慎分析。总的来说，我们的研究为更好地利用这一重要的研究材料提供了一个框架，并充分利用这一资源来提高我们对间日疟原虫生物学的理解。在这项研究中，我们比较了直接从患者身上采集的间日疟原虫与在实验室猴子身上传播的疟原虫的基因组序列。我们发现间日疟原虫在猴子体内的适应和持续繁殖不会引起基因组的系统性变化，因此，这些寄生虫为研究这一重要病原体提供了公正的资源。我们的分析还表明，一些猴子适应间日疟原虫菌株在遗传上不是同质的，在原始患者感染中保留了多种遗传上不同的寄生虫。总的来说，我们的研究证实了猴子适应间日疟原虫菌株在疟疾研究中的效用，但也表明这种资源应该谨慎分析，因为同一菌株的不同样本可能提供不同的生物材料。
Significant insights into the biology of Plasmodium vivax have been gained from the ability to successfully adapt human infections to non-human primates. P. vivax strains grown in monkeys serve as a renewable source of parasites for in vitro and ex vivo experimental studies and functional assays, or for studying in vivo the relapse characteristics, mosquito species compatibilities, drug susceptibility profiles or immune responses towards potential vaccine candidates. Despite the importance of these studies, little is known as to how adaptation to a different host species may influence the genome of P. vivax. In addition, it is unclear whether these monkey-adapted strains consist of a single clonal population of parasites or if they retain the multiclonal complexity commonly observed in field isolates. Here we compare the genome sequences of seven P. vivax strains adapted to New World monkeys with those of six human clinical isolates collected directly in the field. We show that the adaptation of P. vivax parasites to monkey hosts, and their subsequent propagation, did not result in significant modifications of their genome sequence and that these monkey-adapted strains recapitulate the genomic diversity of field isolates. Our analyses also reveal that these strains are not always genetically homogeneous and should be analyzed cautiously. Overall, our study provides a framework to better leverage this important research material and fully utilize this resource for improving our understanding of P. vivax biology. In this study we compare the genome sequences of Plasmodium vivax collected directly from patients with those of parasites propagated in laboratory monkeys. We show that the adaptation and continuous propagation of Plasmodium vivax in monkeys does not induce systematic changes in the genome and, therefore, that these parasites constitute an unbiased resource for studying this important pathogen. Our analyses also reveal that some monkey-adapted Plasmodium vivax strains are not genetically homogenous and retain multiple genetically different parasites present in the original patient infection. Overall, our study confirms the utility of monkey-adapted Plasmodium vivax strains for malaria research but also shows that this resource should be analyzed cautiously as different samples of the same strain might provide different biological material.
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影响因子：
64.8
作者：
POLOGE, LG;RAVETCH, JV
通讯作者：
RAVETCH, JV
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
64.8
作者：
Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
影响因子：
64.5
作者：
CORCORAN, LM;FORSYTH, KP;KEMP, DJ
通讯作者：
KEMP, DJ