DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China.

DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China.
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中国物种丰富的报春属植物DNA条形码评价及其分类学意义

DOI:
10.1371/journal.pone.0122903
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Ge XJ
Ge XJ
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Yan HF;Liu YJ;Xie XF;Zhang CY;Hu CM;Hao G;Ge XJ

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中国东喜马拉雅-横断山脉地区报春花属植物多样性极高,这主要是由于快速的辐射所致。为了克服该属形态分类的困难,我们调查了三个质体区域(rbcL,matK,和trnH-psbA)和两个核标记(ITS和ITS 2),从227个加入代表66报春属物种跨越18个部分,以评估其作为条形码的区分能力。我们发现,ITS单独或与质体区域相结合,表现出最好的歧视,在不同的亚属级和物种水平。建议rbcL + matK + ITS作为目前报春花属植物条形码的首选方法。尽管目前的条形码组合在报春花属许多密切相关的物种中表现不佳,但它仍然为当前报春花属分类提供了许多新的见解,例如潜在的神秘物种的存在,以及一些潜在的不适当的分类处理。DNA条形码技术是报春花属植物综合分类学研究中的一项重要技术,但在一些分类学上具有挑战性的类群中仍需进一步努力提高其有效性。
The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups.
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