DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China.
DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China.
复制标题
中国物种丰富的报春属植物DNA条形码评价及其分类学意义
DOI:
10.1371/journal.pone.0122903
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Ge XJ
中科院分区:
文献类型:
--
作者:
Yan HF;Liu YJ;Xie XF;Zhang CY;Hu CM;Hao G;Ge XJ
The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups.
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DOI:
10.1098/rsbl.2003.0025
发表时间:
2003-08-07
影响因子:
4.7
作者:
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者:
deWaard, JR
影响因子:
3.7
作者:
Huang J;Zhang A;Mao S;Huang Y
通讯作者:
Huang Y
影响因子:
2.2
作者:
Janzen, Daniel H.;Hallwachs, Winnie;Hebert, Paul D. N.
通讯作者:
Hebert, Paul D. N.
影响因子:
2
作者:
Cianchi, R.;Arduino, P.;Bullini, L.
通讯作者:
Bullini, L.
影响因子:
3.7
作者:
Feng J;Jiang D;Shang H;Dong M;Wang G;He X;Zhao C;Mao K
通讯作者:
Mao K