Barcoding poplars (Populus L.) from western China.
Barcoding poplars (Populus L.) from western China.
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来自中国西部的条形码杨树（Populus L.）。
DOI：
10.1371/journal.pone.0071710
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mao K
中科院分区：
文献类型：
--
作者：
Feng J;Jiang D;Shang H;Dong M;Wang G;He X;Zhao C;Mao K
杨树是一个重要的生态和经济树种，但由于广泛的种间杂交和渐渗，以及种内形态变异的高水平，区分野生种相对困难。DNA条形码方法是解决这一问题的一个潜在方案。在这里，我们测试了五个叶绿体条形码和一个核条形码（ITS）的95个树木，代表21个杨树从中国西部。在所有单个条形码候选者中，核ITS的区分能力最高，叶绿体条形码matK（M）、trnG-psbK（G）和psbK-psbI（P）以及trnH-psbA（H）和rbcL（R）的区分能力逐渐降低;核ITS（I）的区分效率也高于叶绿体条形码的任何两个、三个甚至五个位点的组合。在单个叶绿体条形码加核ITS的五种组合中，H+I和P+I分别区分了物种的最高和最低部分。这里检查的条形码或条形码组合的最高鉴别率为55.0%（H+I），并且通常鉴别失败发生在来自同域或近域区域的物种之间。在这个案例研究中，我们表明，当区分来自中国西部的杨属物种时，核ITS区域代表了比任何母系遗传的叶绿体区域或叶绿体区域组合更有前途的条形码。同时，将ITS区与叶绿体区结合起来可能会提高条形码的成功率，并有助于检测最近的种间杂交。未能区分几组杨树物种同域或parapatric地区可能是不完整的谱系排序，频繁的种间杂交和基因渗入的结果。我们同意以前的建议，在植物中构建一个分层的条形码系统，特别是对于具有复杂进化历史的分类群体（如杨）。
Populus is an ecologically and economically important genus of trees, but distinguishing between wild species is relatively difficult due to extensive interspecific hybridization and introgression, and the high level of intraspecific morphological variation. The DNA barcoding approach is a potential solution to this problem. Here, we tested the discrimination power of five chloroplast barcodes and one nuclear barcode (ITS) among 95 trees that represent 21 Populus species from western China. Among all single barcode candidates, the discrimination power is highest for the nuclear ITS, progressively lower for chloroplast barcodes matK (M), trnG-psbK (G) and psbK-psbI (P), and trnH-psbA (H) and rbcL (R); the discrimination efficiency of the nuclear ITS (I) is also higher than any two-, three-, or even the five-locus combination of chloroplast barcodes. Among the five combinations of a single chloroplast barcode plus the nuclear ITS, H+I and P+I differentiated the highest and lowest portion of species, respectively. The highest discrimination rate for the barcodes or barcode combinations examined here is 55.0% (H+I), and usually discrimination failures occurred among species from sympatric or parapatric areas. In this case study, we showed that when discriminating Populus species from western China, the nuclear ITS region represents a more promising barcode than any maternally inherited chloroplast region or combination of chloroplast regions. Meanwhile, combining the ITS region with chloroplast regions may improve the barcoding success rate and assist in detecting recent interspecific hybridizations. Failure to discriminate among several groups of Populus species from sympatric or parapatric areas may have been the result of incomplete lineage sorting, frequent interspecific hybridizations and introgressions. We agree with a previous proposal for constructing a tiered barcoding system in plants, especially for taxonomic groups that have complex evolutionary histories (e.g. Populus).
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