Borrelia burgdorferi genotype predicts the capacity for hematogenous dissemination during early Lyme disease.
Borrelia burgdorferi genotype predicts the capacity for hematogenous dissemination during early Lyme disease.
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DOI：
10.1086/592279
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发表时间：
2008-11-01
期刊：
影响因子：
--
通讯作者：
Schwartz I
中科院分区：
文献类型：
--
作者：
Wormser GP;Brisson D;Liveris D;Hanincová K;Sandigursky S;Nowakowski J;Nadelman RB;Ludin S;Schwartz I
莱姆病是美国最常见的蜱传疾病，在北美仅由严格意义上的伯氏疏螺旋体引起。本研究评估了超过400株B临床分离株的基因型。burgdorferi从患有与游走性红斑相关的早期莱姆病的来自纽约市郊区的患者中恢复;这是迄今为止调查的来自北美的最大数量的疏螺旋体菌株。通过限制性片段长度多态性聚合酶链反应分析16 S-23 S核糖体RNA间隔区和反向线印迹分析外表面蛋白C基因（ospC）进行基因分型。对于一些分离株，还进行了DNA序列分析。结果表明，16 S-23 S核糖体间隔区与ospC之间存在较强的连锁不平衡。大多数B。通过任一分型方法表征的伯氏菌基因型能够在患者中感染和传播。然而，16种ospC基因型中仅4种的独特子集导致>80%的早期播散性莱姆病病例。本研究确定了B。在人类中引起血行性传播的风险最大的伯氏菌基因型。这一信息应考虑在未来的诊断检测和疫苗制剂的发展。
Lyme disease, the most common tickborne disease in the United States, is caused exclusively by Borrelia burgdorferi sensu stricto in North America. The present study evaluated the genotypes of >400 clinical isolates of B. burgdorferi recovered from patients from suburban New York City with early Lyme disease associated with erythema migrans; it is the largest number of borrelial strains from North America ever to be investigated. Genotyping was performed by restriction fragment–length polymorphism polymerase chain reaction analysis of the 16S–23S ribosomal RNA spacer and reverse line blot analysis of the outer surface protein C gene (ospC). For some isolates, DNA sequence analysis was also performed. The findings showed that the 16S–23S ribosomal spacer and ospC are in strong linkage disequilibrium. Most B. burgdorferi genotypes characterized by either typing method were capable of infecting and disseminating in patients. However, a distinct subset of just 4 of the 16 ospC genotypes identified were responsible for >80% of cases of early disseminated Lyme disease. This study identified the B. burgdorferi genotypes that pose the greatest risk of causing hematogenous dissemination in humans. This information should be considered in the future development of diagnostic assays and vaccine preparations.
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DOI：
10.1073/pnas.90.21.10163
发表时间：
1993-11-01
影响因子：
11.1
作者：
DYKHUIZEN, DE;POLIN, DS;LUFT, BJ
通讯作者：
LUFT, BJ
影响因子：
3.1
作者：
Seinost, G;Dykhuizen, DE;Luft, BJ
通讯作者：
Luft, BJ
影响因子：
15.9
作者：
Pal, U;Yang, XF;Fikrig, E
通讯作者：
Fikrig, E
DOI：
10.4269/ajtmh.2008.78.806
发表时间：
2008-05-01
影响因子：
3.3
作者：
Dykhuizen, Daniel E.;Brisson, Dustin;Schwartz, Ira
通讯作者：
Schwartz, Ira
影响因子：
168.9
作者：
Nadelman, RB;Wormser, GP
通讯作者：
Wormser, GP