Genome-Wide Identification of Cyclic Nucleotide-Gated Ion Channel Gene Family in Wheat and Functional Analyses of TaCNGC14 and TaCNGC16.
Genome-Wide Identification of Cyclic Nucleotide-Gated Ion Channel Gene Family in Wheat and Functional Analyses of TaCNGC14 and TaCNGC16.
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小麦中环核苷酸门控离子通道基因家族的全基因组鉴定及 TaCNGC14 和 TaCNGC16 的功能分析
DOI：
10.3389/fpls.2018.00018
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发表时间：
2018
影响因子：
5.6
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Guo J;Islam MA;Lin H;Ji C;Duan Y;Liu P;Zeng Q;Day B;Kang Z;Guo J
环核苷酸门控通道（CNGC）在植物中起着多方面的作用，特别是在与非生物胁迫信号相关的信号传导过程和宿主-病原体相互作用过程中。尽管CNGC家族在植物生存和对环境的响应中起着关键作用，但对普通小麦（Triticum aestivum L.）中CNGC家族的活性和功能知之甚少，是地球仪的重要稳定食物。在这项研究中，我们进行了小麦CNGC家族的全基因组鉴定，共鉴定了47个TaCNGC基因，将这些基因分为四个主要组（I-IV）和两个亚组（IVa和IVb）。序列分析显示存在几个保守的基序，包括磷酸盐结合盒（PBC）和“铰链”区，这两者都被假设为小麦CNGC的功能是至关重要的。在小麦感染Pst，转录水平的TaCNGC 14和TaCNGC 16，这两个成员的组IVb，表现出显着的诱导在兼容的相互作用，而在不兼容的相互作用中观察到的基因表达减少。此外，TaCNGC 14和TaCNGC 16 mRNA的积累显着影响外源施加的激素，包括脱落酸（阿坝），茉莉酸甲酯（MeJA），水杨酸（SA），这表明激素信号和/或感知的作用。TaCNGC 14和TaCNGC 16基因的沉默限制了Pst的生长，提高了小麦对Pst的抗性。本文的结果有助于我们理解小麦CNGC基因家族和TaCNGCs在小麦-Pst互作中的信号转导机制。
Cyclic nucleotide gated channels (CNGCs) play multifaceted roles in plants, particularly with respect to signaling processes associated with abiotic stress signaling and during host-pathogen interactions. Despite key roles during plant survival and response to environment, little is known about the activity and function of CNGC family in common wheat (Triticum aestivum L.), a key stable food around the globe. In this study, we performed a genome-wide identification of CNGC family in wheat and identified a total 47 TaCNGCs in wheat, classifying these genes into four major groups (I–IV) with two sub-groups (IVa and IVb). Sequence analysis revealed the presence of several conserved motifs, including a phosphate binding cassette (PBC) and a “hinge” region, both of which have been hypothesized to be critical for the function of wheat CNGCs. During wheat infection with Pst, the transcript levels of TaCNGC14 and TaCNGC16, both members of group IVb, showed significant induction during a compatible interaction, while a reduction in gene expression was observed in incompatible interactions. In addition, TaCNGC14 and TaCNGC16 mRNA accumulation was significantly influenced by exogenously applied hormones, including abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), suggesting a role in hormone signaling and/or perception. Silencing of TaCNGC14 and TaCNGC16 limited Pst growth and increased wheat resistance against Pst. The results presented herein contribute to our understanding of the wheat CNGC gene family and the mechanism of TaCNGCs signaling during wheat-Pst interaction.
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DOI：
10.1094/mpmi-21-10-1285
发表时间：
2008-10
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Genger RK;Jurkowski GI;McDowell JM;Lu H;Jung HW;Greenberg JT;Bent AF
通讯作者：
Bent AF
小麦过敏诱导反应基因TaHIR1和TaHIR3参与对条锈菌感染和非生物胁迫的反应
影响因子：
6.2
作者：
Duan, Yinghui;Guo, Jun;Kang, Zhensheng
通讯作者：
Kang, Zhensheng
影响因子：
11.6
作者：
Balagué, C;Lin, BQ;Roby, D
通讯作者：
Roby, D
影响因子：
4.9
作者：
Crampton, Bridget G.;Hein, Ingo;Berger, Dave K.
通讯作者：
Berger, Dave K.
影响因子：
11.6
作者：
Ali, Rashid;Ma, Wei;Berkowitz, Gerald A.
通讯作者：
Berkowitz, Gerald A.