Biocompatibility and mineralization potential of new calcium silicate cements.
Biocompatibility and mineralization potential of new calcium silicate cements.
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DOI：
10.1016/j.jds.2022.10.004
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发表时间：
2023-07
影响因子：
3.5
通讯作者：
Shin, Yooseok
中科院分区：
文献类型：
--
作者：
Kim, Byurira;Lee, Yong-Hyuk;Kim, Ik-Hwan;Lee, Ko Eun;Kang, Chung-Min;Lee, Hyo-Seol;Choi, Hyung-Jun;Cheon, Kyounga;Song, Je Seon;Shin, Yooseok
随着硅酸钙骨水泥(CSCs)在各种牙髓治疗中的成功应用，许多新的CSC产品应运而生。本研究的目的是评价新型CSC的生物相容性和矿化潜力。实验材料为NeoMTA Plus和EndoSequence牙根修复材料-Fast Set Puty(ERM-FS)，并与ProRoot MTA进行比较。在体外，评估了新的CSC对干细胞的影响。细胞活力检测、碱性磷酸酶(ALP)检测和钙离子释放检测。活体部分切髓采用裸露牙髓模型。用ProRoot MTA、NeoMTA Plus或ERRM-FS三种材料对36颗牙进行治疗。4周后，拔除牙齿并进行组织学分析。观察牙本质桥形成、牙髓炎症和成牙本质细胞层，并测量各组新形成的钙化屏障面积。三种CSCs在干细胞上表现出相似的细胞活性，并且ALP和钙释放水平在测试材料之间没有显着差异。ProRoot MTA和Erm-FS在钙化屏障质量和牙髓炎症方面显示出比NeoMTA Plus更好的组织愈合过程。测量新形成的钙化区域的结果显示，材料之间没有显著差异。与ProRoot MTA相比，NeoMTA Plus和Erm-FS显示出相似的生物相容性和矿化潜力。因此，这些新的CSC可以作为ProRoot MTA的理想替代品。
As calcium silicate cements (CSCs) have been successfully used in various types of vital pulp therapy, many new CSC products have been developed. The aim of this study was to evaluate the biocompatibilities and mineralization potential of new CSC. The experimental materials were NeoMTA Plus and EndoSequence Root Repair Material-Fast Set Putty (ERRM-FS) which were compared to ProRoot MTA. In vitro, the effects of the new CSC on stem cells were evaluated. Each CSC was prepared for cell viability testing, alkaline phosphatase (ALP) assay, and calcium ion release assay. In vivo, the exposed pulp model was used for the partial pulpotomy procedure. Thirty-six teeth were treated with three materials: ProRoot MTA, NeoMTA Plus, or ERRM-FS. After four weeks, the teeth were extracted and processed for histologic analysis. Dentin bridge formation, pulp inflammation, and odontoblastic cell layer were evaluated and the area of newly formed calcific barrier of each group was measured. Three CSCs demonstrated similar cell viability on stem cells and the levels of ALP and calcium release were not significantly different between tested materials. ProRoot MTA and ERRM-FS showed better tissue healing process than NeoMTA Plus after partial pulpotomy, in terms of quality of calcific barrier and pulp inflammation. The outcomes from measuring newly formed calcific area demonstrated no significant differences between the materials. NeoMTA Plus and ERRM-FS displayed similar biocompatibilities and mineralization potential compared to ProRoot MTA. Therefore, these new CSCs can be used as desirable alternatives to ProRoot MTA.
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影响因子：
3.5
作者：
Kwon W;Kim IH;Kang CM;Kim B;Shin Y;Song JS
通讯作者：
Song JS
影响因子：
5
作者：
Aeinehchi, M;Eslami, B;Saffar, AS
通讯作者：
Saffar, AS
影响因子：
4.6
作者：
Santiago MC;Gomes-Cornélio AL;de Oliveira LA;Tanomaru-Filho M;Salles LP
通讯作者：
Salles LP
影响因子：
4.1
作者：
Papagerakis, P;Berdal, A;Macdougall, M
通讯作者：
Macdougall, M
影响因子：
1.8
作者：
Dahake, Prasanna T.;Panpaliya, Nikita P.;Bogar, Chetana
通讯作者：
Bogar, Chetana