Experimental repetitive mild traumatic brain injury induces deficits in trabecular bone microarchitecture and strength in mice.
Experimental repetitive mild traumatic brain injury induces deficits in trabecular bone microarchitecture and strength in mice.
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DOI：
10.1038/boneres.2017.42
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发表时间：
2017
期刊：
影响因子：
12.7
通讯作者：
Mohan S
中科院分区：
文献类型：
--
作者：
Kesavan C;Bajwa NM;Watt H;Mohan S
为了评估重复性轻度创伤性脑损伤（mTBI）对骨骼的长期后果，使用重量跌落模型在10周龄雌性C57 BL/6 J小鼠中诱导mTBI，每天一次，连续4天，在不同的跌落高度（0.5、1和1.5 m），并在撞击后的不同时间点评估骨骼表型。在撞击后2、8和12周，胫骨干骺端的体内显微CT（μ-CT）分析显示骨小梁质量减少5%-32%。组织形态学分析表明，在1.5米的影响小鼠在12周后的影响，在继发性海绵骨形成率降低。撞击后2周和8周，1.5 m跌落高度组胫骨近端干骺端的表观模量（骨强度）降低了30%（P<0.05）。第五腰椎的离体μ-CT分析显示，在所有三个跌落高度组中，12周龄时松质骨质量显著降低。在撞击后2周，0.5、1.0和1.5 m坠落高度组的血清骨钙素水平分别下降了22%、15%和19%。在撞击后2周，与对照1小鼠相比，mTBI小鼠的血清IGF-I水平降低了18%-32%。血清骨钙素和IGF-I水平与骨小梁BV/TV相关（r2=0.14和0.16，P<0.05）。总之，重复性mTBI通过降低内分泌IGF-I作用影响成骨细胞功能，对骨小梁微结构和骨机械性能产生显著的负面影响。
To evaluate the long-term consequence of repetitive mild traumatic brain injury (mTBI) on bone, mTBI was induced in 10-week-old female C57BL/6J mice using a weight drop model, once per day for 4 consecutive days at different drop heights (0.5, 1 and 1.5 m) and the skeletal phenotype was evaluated at different time points after the impact. In vivo micro-CT (μ-CT) analysis of the tibial metaphysis at 2, 8 and 12 weeks after the impact revealed a 5%–32% reduction in trabecular bone mass. Histomorphometric analyses showed a reduced bone formation rate in the secondary spongiosa of 1.5 m impacted mice at 12 weeks post impact. Apparent modulus (bone strength), was reduced by 30% (P<0.05) at the proximal tibial metaphysis in the 1.5 m drop height group at 2 and 8 weeks post impact. Ex vivo μ-CT analysis of the fifth lumbar vertebra revealed a significant reduction in trabecular bone mass at 12 weeks of age in all three drop height groups. Serum levels of osteocalcin were decreased by 22%, 15%, and 19% in the 0.5, 1.0 and 1.5 m drop height groups, respectively, at 2 weeks post impact. Serum IGF-I levels were reduced by 18%–32% in mTBI mice compared to contro1 mice at 2 weeks post impact. Serum osteocalcin and IGF-I levels correlated with trabecular BV/TV (r2=0.14 and 0.16, P<0.05). In conclusion, repetitive mTBI exerts significant negative effects on the trabecular bone microarchitecture and bone mechanical properties by influencing osteoblast function via reduced endocrine IGF-I actions.
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影响因子：
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作者：
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