A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3
A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3
复制标题
使用 Nanos3 鉴定了橄榄鲽 (Paralichthys olivaceus) 原始生殖细胞迁移的新模式
DOI：
10.1007/s00427-015-0503-6
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发表时间：
2015-05
影响因子：
2.4
通讯作者：
Zou, Yuxia
中科院分区：
文献类型：
--
作者：
Wang, Qian;Wu, Zhihao;You Feng;Zou, Yuxia
橄榄(Paralichthys Olivaceus)是一种重要的海水养殖鱼类。然而，关于原始生殖细胞(PGC)在该物种中的发育和迁移的信息很少；这些信息对于在人工繁殖中的应用和遗传资源的保存至关重要。在这里，我们试图通过分离生殖系特异性基因nanos3并分析其在橄榄比目鱼中的表达来弥补这一信息缺失。序列分析表明，橄榄比目鱼Nanos3含有一个典型的RNA结合的锌指结构域。系统发育分析表明，橄榄比目鱼的Nanos3与巴鳍比目鱼(Verasper Moseri)的Nanos3聚在一起。在比目鱼中，Nanos3在胚胎发育过程中持续表达。整装原位杂交结果表明，橄榄PGC存在一种新的迁移模式，它结合了青鱼、鲱鱼和虎鱼的PGC迁移模式的元素。在牙鲆胚胎发育早期，原生殖细胞沿着侧板中胚层排列成两条松散、细长的线条，中期聚集成一个松散的簇状，在体细胞发生后期重新排列成两个紧密的簇状，最后以两簇的形式迁移到生殖脊。此外，整体原位杂交显示，基质衍生因子1(SDF1)的表达对PGC在体细胞发生过程中的迁移具有重要的指导作用。具体地说，在胚胎发生中期，SDF1将两个细长品系的原生殖细胞聚集成一个松散的簇状。此外，通过注射含有绿色荧光蛋白(GFP)和橄榄牙鲨Nanos3‘非翻译区的嵌合RNA，成功地显示了斑马鱼中的PGCs。这些发现为了解PGC在橄榄比目鱼中的迁移和发育提供了新的见解，也将促进该物种的生殖细胞操作。
The olive flounder (Paralichthys olivaceus) is an important cultured marine fish. However, little information is available on primordial germ cell (PGC) development and migration in this species; such information is vital for applications in artificial reproduction and for the preservation of genetic resources. Here, we sought to remedy this information deficit by isolating the germline-specific gene nanos3 and analyzing its expression in olive flounder. Sequencing analysis showed that olive flounder nanos3 contained a typical RNA-binding zinc finger domain. A phylogenetic analysis demonstrated that nanos3 of the olive flounder grouped with that of the barfin flounder (Verasper moseri). In the olive flounder, nanos3 was consistently expressed during embryogenesis. Whole-mount in situ hybridization showed that a new pattern of PGC migration was present in olive flounder, which combined elements of the PGC migration patterns of medaka, herring, and goby. In olive flounder, PGCs aligned along the lateral plate mesoderm in two loose, elongated lines at early embryogenesis, aggregated into a single loose cluster at mid-embryogenesis, then re-aligned into two tight clusters at late somitogenesis, and finally migrated to the genital ridge as two clusters. Furthermore, whole-mount in situ hybridization revealed that expression of stromal derived factor 1 (Sdf1) was important for guiding of PGC migration during somitogenesis. In particular, Sdf1 directed aggregation of PGCs into a single loose cluster from the two elongated lines during mid-embryogenesis. Additionally, PGCs in zebrafish were successfully visualized by injection of chimeric RNA containing the green fluorescent protein (GFP) and 3′ untranslated region of olive flounder nanos3. These findings provide new insights into PGC migration and development in olive flounder and will also facilitate germ cell manipulation in this species.
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影响因子：
3
作者：
Adrijana Skugor;Krasimir Slanchev;J. Torgersen;H. Tveiten;Ø. Andersen
通讯作者：
Adrijana Skugor;Krasimir Slanchev;J. Torgersen;H. Tveiten;Ø. Andersen
影响因子：
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影响因子：
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作者：
Saito T;Goto-Kazeto R;Kawakami Y;Nomura K;Tanaka H;Adachi S;Arai K;Yamaha E
通讯作者：
Yamaha E
影响因子：
3
作者：
Zhang, YQ;Tan, XA;Zhang, PJ;Xu, YL
通讯作者：
Xu, YL