The mechanism for primordial germ-cell migration is conserved between Japanese eel and zebrafish.
The mechanism for primordial germ-cell migration is conserved between Japanese eel and zebrafish.
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DOI：
10.1371/journal.pone.0024460
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Yamaha E
中科院分区：
文献类型：
--
作者：
Saito T;Goto-Kazeto R;Kawakami Y;Nomura K;Tanaka H;Adachi S;Arai K;Yamaha E
原始生殖细胞（PGCs）在早期发育过程中从体细胞中分离出来。这些细胞在其他地方产生，必须穿过胚胎迁移到发育中的性腺前体。与PGC迁移相关的几种分子（即死端，nanos1和cxcr4）在门的边界上高度保守。然而，由于细胞迁移是一个复杂的过程，在空间和时间上受到多个衔接子和信号效应子的调节，因此该过程不太可能仅用这些已知基因来解释。事实上，已经表明，在硬骨鱼类物种之间的发育过程中，PGC迁移模式存在差异。然而，目前还不清楚PGC迁移的实际机制是否在物种之间是保守的。本研究通过将日本鳗鲡（Anguilla japonica）PGCs移植到斑马鱼（Danio rerio）胚胎中，研究了日本鳗鲡（Anguilla japonica）胚胎PGCs的迁移，并探讨了日本鳗鲡和斑马鱼之间的迁移机制。实验结果表明，鳗鱼PGCs可随内源PGCs沿着向斑马鱼胚胎性腺区迁移，但不同种类的鳗鱼PGCs迁移方式、行为和定居点有所不同。我们的研究结果表明，在胚胎发育过程中的PGCs的迁移机制是高度保守的这两个远亲物种（属于不同的硬骨鱼目）。
Primordial germ cells (PGCs) are segregated and specified from somatic cells during early development. These cells arise elsewhere and have to migrate across the embryo to reach developing gonadal precursors. Several molecules associated with PGC migration (i.e. dead-end, nanos1, and cxcr4) are highly conserved across phylum boundaries. However, since cell migration is a complicated process that is regulated spatially and temporally by multiple adaptors and signal effectors, the process is unlikely to be explained by these known genes only. Indeed, it has been shown that there are variations in PGC migration pattern during development among teleost species. However, it is still unclear whether the actual mechanism of PGC migration is conserved among species. In this study, we studied the migration of PGCs in Japanese eel (Anguilla japonica) embryos and tested the migration mechanism between Japanese eel and zebrafish (Danio rerio) for conservation, by transplanting eel PGCs into zebrafish embryos. The experiments showed that eel PGCs can migrate toward the gonadal region of zebrafish embryos along with endogenous PGCs, even though the migration patterns, behaviors, and settlements of PGCs are somewhat different between these species. Our results demonstrate that the migration mechanism of PGCs during embryonic development is highly conserved between these two distantly related species (belonging to different teleost orders).
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影响因子：
3.3
作者：
Inoue JG;Miya M;Miller MJ;Sado T;Hanel R;Hatooka K;Aoyama J;Minegishi Y;Nishida M;Tsukamoto K
通讯作者：
Tsukamoto K
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作者：
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通讯作者：
SCHILLING, TF
影响因子：
3.7
作者：
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通讯作者：
Strüssmann CA