TiO(2)-DNA Nanosensor In Situ for Quick Detection of Nasal Flora in Allergic Rhinitis Patients.
TiO(2)-DNA Nanosensor In Situ for Quick Detection of Nasal Flora in Allergic Rhinitis Patients.
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DOI：
10.1155/2022/5708326
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发表时间：
2022
影响因子：
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通讯作者：
中科院分区：
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相关研究发现，变应性鼻炎（AR）可能与鼻腔植物群失衡有关。因此，如果能快速检测出AR患者鼻腔植物群，对研究AR患者鼻腔植物群的分布规律，探讨其与AR的相关性具有重要意义。 设计一种新型、方便的纳米DNA传感器，用于快速筛查变应性鼻炎（AR）患者鼻腔植物群，为AR的防治提供实验依据。 我们合成了一种纳米结构的DNA生物传感器Nano-TiO 2-DNA传感器，它可以与鼻粘膜或分泌物样品高效结合，原位检测某些植物群，而无需DNA提取或RNA测序。在物理性能测试中，首先测试了纳米TiO 2的渗透性、溶解性和储存温度，为纳米TiO 2-DNA传感器的合成提供实验依据。随后，进一步测试了纳米TiO 2-DNA传感器对金黄色葡萄球菌的渗透性。在一项临床实验中，我们选取了2020年9月至2021年9月在我院治疗的60例AR患者作为AR组，同时选取了60例健康体检者作为对照组。采用纳米TiO 2-DNA传感器检测变应性鼻炎患者鼻腔典型植物群，Pearson相关分析探讨鼻腔植物群与血清IgE、嗜酸性粒细胞的相关性。 在理化特性方面，该传感器可直接特异性地渗透到特定的细菌中。它与靶具有高亲和能力，并且可以通过定性和定量评估释放的荧光来检测该组合。它可以在-20 °C的乙醇中稳定储存。通过这种传感器，我们成功地检测出了金黄色葡萄球菌，肺炎克雷伯菌，草绿色链球菌在AR患者与健康人相比，这将有助于这些患者在预防急性鼻窦炎和急性或亚急性肺炎。变形杆菌与AR的正相关性最强，放线菌与AR的负相关性最强。 Nano-TiO 2-DNA传感器将帮助门诊医生更快速地筛查AR患者的某些鼻腔植物群，并改善AR相关并发症的预防。
A relevant study found that allergic rhinitis (AR) may be related to the imbalance of nasal flora. Therefore, if the nasal flora of AR patients can be detected quickly, it is of great significance to study the distribution law of nasal flora in AR patients and explore its correlation with AR. To design a new and convenient nano-DNA sensor for quick screening of nasal flora in allergic rhinitis (AR) patients, so as to provide experimental basis for the prevention and treatment of AR. We create a synthesized nanostructured DNA biosensor called Nano-TiO2-DNA sensor which can be combined with samples from nasal mucosa or secretion with high efficiency and detect certain flora in situ without DNA extraction or RNA sequencing. In a physical property test, firstly, we tested the permeability, solubility, and storage temperature of nano-TiO2, so as to provide experimental basis for the synthesis of Nano-TiO2-DNA sensor. Subsequently, the permeability of Nano-TiO2-DNA sensor in Staphylococcus aureus was further tested. In a clinical experiment, we selected 60 AR patients treated in our hospital from September 2020 to September 2021 as the AR group and 60 healthy people who underwent physical examination at the same time as the control group. The Nano-TiO2-DNA sensor was used to detect typical nasal flora in AR patients, and Pearson's correlation analysis was used to explore the correlation between nasal flora with serum IgE and eosinophils. As for physicochemical characteristics, this sensor can permeate into certain bacteria directly and specifically. It has high affinity ability with a target, and the combination can be detected by evaluating the released fluorescence qualitatively and quantitatively. It can be stored at −20°C in ethyl alcohol stably. By this sensor, we have successfully detected Staphylococcus aureus, Klebsiella pneumoniae, and viridans streptococci in AR patients compared with healthy people, which will help these patients in the prevention of acute sinusitis and acute or subacute pneumonia. Furthermore, we found Proteus had the strongest positive correlation with AR while Actinomyces had the biggest negative correlation. The Nano-TiO2-DNA sensor will help an outpatient doctor more for quick screening certain nasal flora in AR patients and improve the prevention of AR-related complications.
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DOI：
10.1186/s12948-017-0070-7
发表时间：
2017-01-01
期刊：
Clinical and molecular allergy : CMA
影响因子：
--
作者：
Martignago, Irene;Incorvaia, Cristoforo;Ridolo, Erminia
通讯作者：
Ridolo, Erminia
影响因子：
14.2
作者：
Dykewicz, Mark S.;Hamilos, Daniel L.
通讯作者：
Hamilos, Daniel L.
影响因子：
1.8
作者：
Bolat, E.;Arikoglu, T.;Kuyucu, S.
通讯作者：
Kuyucu, S.
影响因子：
2.8
作者：
Korosec, Peter;Silar, Mira;Kosnik, Mitja
通讯作者：
Kosnik, Mitja
影响因子：
4.6
作者：
Yamamoto, K.;Kawamura, I.;Mitsuyama, M.
通讯作者：
Mitsuyama, M.