Analysis of the p53 gene in relation to tobacco and alcohol in cancers of the upper aero‐digestive tract
Analysis of the p53 gene in relation to tobacco and alcohol in cancers of the upper aero‐digestive tract
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上消化道癌症中与烟草和酒精相关的 p53 基因分析
DOI：
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发表时间：
1995
影响因子：
6.4
通讯作者：
M. Boiocchi
中科院分区：
文献类型：
--
作者：
S. Franceschi;A. Gloghini;R. Maestro;L. Barzan;E. Bidoli;R. Talamini;T. Vukosavljevic;A. Carbone;M. Boiocchi
为阐明P53基因表达与吸烟、饮酒习惯的关系，从1986年至1991年在意大利东北部波德诺内省进行的病例对照调查中抽取上消化道癌患者135例。83例受试者有足够的病理资料进行免疫组织化学分析。根据免疫反应强度和P53阳性细胞百分率对P53表达水平进行分类。用单链构象多态和测序的方法分析了8例p53阳性的终生非吸烟者在不同酒精摄入量下的突变情况。通过优势比和95%可信区间来评估P53表达与吸烟和饮酒之间的关系。值得注意的是，男性(39/65)高于女性(6/18)。未发现随着吸烟和饮酒水平的增加，P53阳性癌症的百分比增加的显著趋势，特别是在考虑性别因素之后。对于特定的突变模式，在8例终生不吸烟的饮酒者中，有6例突变涉及G：C碱基对。4例发现G-A转换。目前的研究不支持P53表达升高与吸烟或饮酒之间的联系。它提供了一个在大规模流行病学调查框架内进行分子生物学研究的例子。
To elucidate the relationship, if any, of p53 gene expression according to smoking and drinking habits, 135 subjects with cancer of the upper aero‐digestive tract were sampled from a case‐control investigation conducted in Pordenone province, Northeast Italy, between 1986 and 1991. Adequate pathological material for immunohistochemical analysis was available for 83 subjects. Level of p53 expression was classified according to intensity on immunoreaction and percentage of p53‐positive cells. Mutations were analyzed by means of single‐strand conformation polymorphism and sequencing in 8 p53‐positive life‐long non‐smokers exposed to various levels of alcohol intake. The association between p53 expression and smoking status and alcohol intake was evaluated by means of odds ratios, and 95% confidence intervals. Significantly, more men (39/65) than women (6/18) had elevated p53 expression. No significant trend of increasing percentage of p53‐positive cancers with increasing smoking and drinking level was detected, especially after allowance for gender. With respect to specific mutation pattern, in 8 life‐long non smokers who drank alcohol, 6 mutations involved G:C base pairs. G‐to‐A transitions were identified in 4 cases. The present study does not support an association between elevated p53 expression and tobacco smoking or alcohol intake. It provides an example of a molecular biology study in the framework of a large epidemiological investigation.
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酒精与癌症。
影响因子：
--
作者：
Rogers,AE;Conner,MW
通讯作者：
Conner,MW
影响因子：
8
作者：
R. Maestro;R. Dolcetti;D. Gasparotto;C. Doglioni;S. Pelucchi;L. Barzan;E. Grandi;M. Boiocchi
通讯作者：
R. Maestro;R. Dolcetti;D. Gasparotto;C. Doglioni;S. Pelucchi;L. Barzan;E. Grandi;M. Boiocchi
影响因子：
56.9
作者：
HOLLSTEIN, M;SIDRANSKY, D;HARRIS, CC
通讯作者：
HARRIS, CC
影响因子：
10.4
作者：
Monchaux G;Morlier JP;Morin M;Chameaud J;Lafuma J;Masse R
通讯作者：
Masse R