Molecular characterization and immunolocalization of the olfactory co-receptor Orco from two blood-feeding muscid flies, the stable fly (Stomoxys calcitrans, L.) and the horn fly (Haematobia irritans irritans, L.).
Molecular characterization and immunolocalization of the olfactory co-receptor Orco from two blood-feeding muscid flies, the stable fly (Stomoxys calcitrans, L.) and the horn fly (Haematobia irritans irritans, L.).
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DOI：
10.1111/imb.12009
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发表时间：
2013-04
影响因子：
2.6
通讯作者：
Olafson PU
中科院分区：
文献类型：
--
作者：
Olafson PU
咬蝇是具有重要经济意义的吸血害虫，具有医学和兽医意义。基于化学感觉的咬蝇行为，如宿主/营养源定位和产卵位点选择，是开发补充控制策略的有趣目标。为了扩大我们对叮蝇化学感觉通路的理解，从稳定蝇（Scal\Orco）和角蝇（Hirr\Orco）两种具有代表性的叮蝇物种中分离出编码高度保守的昆虫气味共受体（Orco）的转录本。Orco与气味特异性气味受体形成复合物，形成气味门控离子通道。据预测，叮咬蝇转录本编码的蛋白质与已发表的昆虫Orco序列具有87% - 94%的氨基酸相似性，并且在不同的未成熟阶段以及与嗅觉相关的成年结构（即触角和上颌触须）和味觉（即喙）中都有检测到。此外，利用针对两种蝇之间保守的肽序列的抗血清，将相关蛋白免疫定位到特定的触角感受器上。本研究结果为对尚不明确的稳定蝇和角蝇常规气味受体的驱避/引诱效应的功能评价提供了依据。
Biting flies are economically important, blood-feeding pests of medical and veterinary significance. Chemosensory-based biting fly behaviors, such as host/nutrient source localization and ovipositional site selection, are intriguing targets for the development of supplemental control strategies. In an effort to expand our understanding of biting fly chemosensory pathways, transcripts encoding the highly conserved insect odorant co-receptor (Orco) were isolated from two representative biting fly species, the stable fly (Scal\Orco) and the horn fly (Hirr\Orco). Orco forms a complex with an odor-specific odorant receptor to form an odor-gated ion channel. The biting fly transcripts were predicted to encode proteins with 87% – 94% amino acid similarity to published insect Orco sequences and were detected in various immature stages as well as in adult structures associated with olfaction, i.e. antennae and maxillary palps, and gustation, i.e. proboscis. Further, the relevant proteins were immunolocalized to specific antennal sensilla using anti-serum raised against a peptide sequence conserved between the two fly species. Results from this study provide a basis for functional evaluation of repellent/attractant effects on as yet uncharacterized stable fly and horn fly conventional odorant receptors.
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DOI：
10.1111/j.1460-9568.2007.05786.x
发表时间：
2007-09
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Ghaninia M;Ignell R;Hansson BS
通讯作者：
Hansson BS
影响因子：
9.8
作者：
Benton, Richard;Sachse, Silke;Michnick, Stephen W;Vosshall, Leslie B
通讯作者：
Vosshall, Leslie B
影响因子：
1.9
作者：
Birkett, MA;Agelopoulos, N;Woodcock, CM
通讯作者：
Woodcock, CM
DOI：
10.1093/aesa/58.6.909
发表时间：
1965-01-01
期刊：
ANN ENTOMOL SOC AMER
影响因子：
--
作者：
ADAMS, JEAN R.;HOLBERT, PAULINE E.;FORGASH, ANDREW J.
通讯作者：
FORGASH, ANDREW J.
影响因子：
2.1
作者：
Cupp, EW;Cupp, MS;Kunz, SE
通讯作者：
Kunz, SE