Functional classification and central nervous projections of olfactory receptor neurons housed in antennal trichoid sensilla of female yellow fever mosquitoes, Aedes aegypti.
Functional classification and central nervous projections of olfactory receptor neurons housed in antennal trichoid sensilla of female yellow fever mosquitoes, Aedes aegypti.
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DOI：
10.1111/j.1460-9568.2007.05786.x
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发表时间：
2007-09
期刊：
影响因子：
--
通讯作者：
Hansson BS
中科院分区：
文献类型：
--
作者：
Ghaninia M;Ignell R;Hansson BS
蚊子高度依赖于它们的嗅觉系统，例如宿主位置和花蜜喂养和产卵地点的识别。气味是由嗅觉受体神经元（ORN）检测到的，这些神经元位于触角和下颚须上的毛状结构、感受器中。为了阐明嗅觉系统在黄热病媒介埃及伊蚊中的功能，我们对雌性触角上的触角感器进行了单感器记录。这些感器分为四种不同的形态类型。基于对一组16种气味化合物的反应，我们确定了18种不同的ORN类型，分布在10种感器类型中。的ORN响应行为相关的嗅觉线索，如产卵引诱剂和汗传化合物，包括4-甲基环己醇和吲哚，分别。安置在这些感器中的两个ORN，以及安置在另外的感器类型中的两个ORN，对任何测试的化合物都没有反应。安置在个人感器的ORN表现出刻板的配对，并显示信号模式（兴奋性和抑制性）以及在时间响应模式的差异。除了生理特性，我们进行了顺行神经生物素染色的功能鉴定的ORN，以确定在初级嗅觉中心，触角叶的嗅球之间的功能地图。将靶向肾小球与已建立的3D图进行比较。我们的数据表明，ORN类型的轴突发送到定义的触角叶肾小球在一个刻板的模式。
Mosquitoes are highly dependent on their olfactory system for, e.g. host location and identification of nectar-feeding and oviposition sites. Odours are detected by olfactory receptor neurons (ORNs) housed in hair-shaped structures, sensilla, on the antennae and maxillary palps. In order to unravel the function of the olfactory system in the yellow fever vector, Aedes aegypti, we performed single-sensillum recordings from trichoid sensilla on female antennae. These sensilla are divided into four distinct morphological types. Based on the response to a set of 16 odour compounds, we identified 18 different ORN types, housed in 10 sensillum types. The ORNs responded to behaviourally relevant olfactory cues, such as oviposition attractants and sweat-borne compounds, including 4-methylcyclohexanol and indole, respectively. Two ORNs housed in these sensilla, as well as two ORNs housed in an additional sensillum type, did not respond to any of the compounds tested. The ORNs housed in individual sensilla exhibited stereotypical pairing and displayed differences in signalling mode (excitatory and inhibitory) as well as in temporal response patterns. In addition to physiological characterization, we performed anterograde neurobiotin stainings of functionally identified ORNs in order to define the functional map among olfactory glomeruli in the primary olfactory centre, the antennal lobe. The targeted glomeruli were compared with an established 3D map. Our data showed that the ORN types sent their axons to defined antennal lobe glomeruli in a stereotypic pattern.
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影响因子：
7.8
作者：
Knuesel, Philipp;Carlsson, Mikael A.;Verschure, Paul F. M. J.
通讯作者：
Verschure, Paul F. M. J.
DOI：
10.1073/pnas.0601107103
发表时间：
2006-09-05
影响因子：
11.1
作者：
Kwon, Hyung-Wook;Lu, Tan;Zwiebel, Laurence J.
通讯作者：
Zwiebel, Laurence J.
影响因子：
1.5
作者：
DENOTTER, CJ;VANNATERS, WMV
通讯作者：
VANNATERS, WMV
DOI：
10.1007/bf01380052
发表时间：
1976-01-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY
影响因子：
--
作者：
DAVIS, EE;SOKOLOVE, PG
通讯作者：
SOKOLOVE, PG
影响因子：
2.1
作者：
BENTLEY, MD;MCDANIEL, IN;DAVIS, EE
通讯作者：
DAVIS, EE