Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing.
Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing.
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DOI：
10.1021/acs.analchem.9b04542
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发表时间：
2020-01-07
影响因子：
7.4
通讯作者：
Fernandez-Lima F
中科院分区：
文献类型：
--
作者：
Castellanos A;Hernandez MG;Tomic-Canic M;Jozic I;Fernandez-Lima F
皮肤修复是人类健康的重要方面。虽然健康角质层和表皮的组成是普遍理解的，但在皮肤修复过程中分子组分的动员在很大程度上仍然未知。在目前的工作中，我们利用多模式，原位，质谱和免疫荧光成像的表征新形成的表皮以下的第一个96小时的上皮形成的最初急性伤口。特别是，TOF-SIMS和确证性MALDI FT-ICR MS（/MS）分析允许绘制几种脂质类别，包括磷脂、中性脂质、胆固醇、神经酰胺和游离脂肪酸。内源性脂质物质位于离散的表皮皮肤层中，包括角质层（SC）、颗粒层（SG）、基底层（SB）和真皮。实验表明，健康的再上皮化皮肤的特征在于沿着角质层朝向迁移的上皮舌沿着减少的胆固醇硫酸盐信号。报告了硫酸胆固醇的空间分布和相对丰度，并与愈合时间相关。多模态成像方法使得能够基于分子组分的精确质量和碎片模式进行原位高置信度化学映射。使用来自相同组织的分析后免疫荧光成像证实了内源性脂质物质在高空间分辨率（约几微米）下的定位。
Skin repair is a significant aspect of human health. While the makeup of healthy stratum corneum and epidermis is generally understood, the mobilization of molecular components during skin repair remains largely unknown. In the present work, we utilize multi-modal, in-situ, mass spectrometry and immunofluorescence imaging for the characterization of newly formed epidermis following an initial acute wound for the first 96h of epithelization. In particular, TOF-SIMS and confirmatory MALDI FT-ICR MS (/MS) analysis permitted the mapping of several lipid classes including phospholipids, neutral lipids, cholesterol, ceramides, and free fatty acids. Endogenous lipid species were localized in discrete epidermal skin layers, including the stratum corneum (SC), stratum granulosum (SG), stratum basale (SB), and dermis. Experiments revealed that healthy re-epithelializing skin is characterized by diminished cholesterol sulfate signal along the stratum corneum towards the migrating epithelial tongue. The spatial distribution and relative abundances of cholesterol sulfate are reported and correlated with the healing time. The multi-modal imaging approach enabled in-situ high-confidence chemical mapping based on accurate mass and fragmentation pattern of molecular components. The use of post-analysis immunofluorescence imaging from the same tissue confirmed the localization of endogenous lipid species at high spatial resolution (~ few microns).
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影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
7.4
作者：
Malm, Jakob;Giannaras, Dimitrios;Sjovall, Peter
通讯作者：
Sjovall, Peter
影响因子：
2.1
作者：
Kezutyte, Toma;Desbenoit, Nicolas;Briedis, Vitalis
通讯作者：
Briedis, Vitalis
DOI：
10.1016/j.bbalip.2019.04.005
发表时间：
2019-08-01
影响因子：
4.8
作者：
Berkers, T.;Boiten, W. A.;Bouwstra, J. A.
通讯作者：
Bouwstra, J. A.
影响因子：
4.3
作者：
Bonnel, David;Legouffe, Raphael;Nielsen, Kim T.
通讯作者：
Nielsen, Kim T.