Strategies for Highly Efficient Rabbit Sperm Cryopreservation.
Strategies for Highly Efficient Rabbit Sperm Cryopreservation.
复制标题
高效兔精子冷冻保存策略
DOI：
10.3390/ani11051220
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发表时间：
2021-04-23
期刊：
影响因子：
--
通讯作者：
Fan J
中科院分区：
文献类型：
--
作者：
Nishijima K;Kitajima S;Matsuhisa F;Niimi M;Wang CC;Fan J
兔子在经济和生物医学上都是一种有价值的动物。因此，保存许多家兔品系是至关重要的。迄今为止，精子冷冻保存是保存兔系最有效的方法之一，因为它很容易从单个雄性反复收集射精并对多个雌性进行人工授精。尽管这种方法被广泛使用，但精子的冷却、冷冻和解冻过程仍存在一些问题，这些问题明显影响保存精子的质量。在本文中，我们将回顾近年来在兔精子冷冻保存方面取得的进展，并讨论可能影响精子损伤的因素，包括冷冻扩展剂、冷冻保护剂、补充剂和程序。摘要兔子在经济和生物医学上都是一种有价值的动物。精子冷冻保存是保存家兔品系最有效的方法之一，因为它可以很容易地从单个雄性反复收集射精并人工授精到多个雌性。在精子的冷却、冷冻和解冻过程中，渗透胁迫、冷休克、胞内冰晶的形成和活性氧的过量产生会破坏精子的质膜、细胞质和基因组结构。在这篇综述中，我们将讨论近年来在减少兔精子细胞损伤方面所取得的进展，包括冷冻扩展剂、冷冻保护剂、补充剂和程序。
Simple Summary The rabbit is a valuable animal for both the economy and biomedical sciences. Therefore, the preservation of many rabbit strains is vitally important. So far, sperm cryopreservation is one of the most efficient ways to preserve rabbit strains because it is easy to collect ejaculate repeatedly from a single male and perform artificial insemination to multiple females. Although this method is widely used, there are still some concerns regarding the cooling, freezing and thawing process of sperms, which markedly affects the quality of preserved sperms. In this article, we will review the progress made during the past years in terms of cryopreservation of rabbit sperms and discuss those factors that would possibly influence sperm damage including freezing extender, cryoprotectant, supplements, and procedures. Abstract The rabbit is a valuable animal for both the economy and biomedical sciences. Sperm cryopreservation is one of the most efficient ways to preserve rabbit strains because it is easy to collect ejaculate repeatedly from a single male and inseminate artificially into multiple females. During the cooling, freezing and thawing process of sperms, the plasma membrane, cytoplasm and genome structures could be damaged by osmotic stress, cold shock, intracellular ice crystal formation, and excessive production of reactive oxygen species. In this review, we will discuss the progress made during the past years regarding efforts to minimize the cell damage in rabbit sperms, including freezing extender, cryoprotectants, supplements, and procedures.
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影响因子：
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