Interaction of BES1 and LBD37 transcription factors modulates brassinosteroid-regulated root forging response under low nitrogen in arabidopsis.
Interaction of BES1 and LBD37 transcription factors modulates brassinosteroid-regulated root forging response under low nitrogen in arabidopsis.
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DOI：
10.3389/fpls.2022.998961
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
维生素C在植物生长发育和环境响应中起着重要的调节作用。BR信号通过控制BES 1/BZR 1调节子的活性来调节多种生物学过程。BR除了促进植物生长外，还参与了低氮条件下根系觅食反应的调控，但BR信号如何调控这一过程尚不清楚。在这里，我们表明，BES 1和LBD 37拮抗调节根觅食反应在低氮条件下。低氮对BES 1基因的转录水平和去磷酸化水平都有显著的诱导作用，且在根中的诱导作用最明显。表型分析表明，BES 1功能获得突变体和过表达转基因植株在低氮胁迫下表现出侧根的生长，BES 1负调控硝酸盐信号途径的阻遏物，正调控NO3 -吸收和信号传导所需的几个关键基因。相反，BES 1基因敲除突变体BES 1-RNAi表现出显著降低侧根伸长响应低N。此外，我们确定了一个BES 1相互作用蛋白，LBD 37，这是一个负阻遏N可用性信号。我们的结果表明，BES 1可以抑制LBD 37对N-反应基因的转录抑制。因此，我们的研究结果表明，BES 1-LBD 37模块作为关键节点整合BR信号和氮信号调节根锻造反应在LN条件下。
Brassinosteriod (BR) plays important roles in regulation of plant growth, development and environmental responses. BR signaling regulates multiple biological processes through controlling the activity of BES1/BZR1 regulators. Apart from the roles in the promotion of plant growth, BR is also involved in regulation of the root foraging response under low nitrogen, however how BR signaling regulate this process remains unclear. Here we show that BES1 and LBD37 antagonistically regulate root foraging response under low nitrogen conditions. Both the transcriptional level and dephosphorylated level of BES1, is significant induced by low nitrogen, predominantly in root. Phenotypic analysis showed that BES1 gain-of-function mutant or BES1 overexpression transgenic plants exhibits progressive outgrowth of lateral root in response to low nitrogen and BES1 negatively regulates repressors of nitrate signaling pathway and positively regulates several key genes required for NO3 - uptake and signaling. In contrast, BES1 knock-down mutant BES1-RNAi exhibited a dramatical reduction of lateral root elongation in response to low N. Furthermore, we identified a BES1 interacting protein, LBD37, which is a negative repressor of N availability signals. Our results showed that BES1 can inhibit LBD37 transcriptional repression on N-responsive genes. Our results thus demonstrated that BES1-LBD37 module acts critical nodes to integrate BR signaling and nitrogen signaling to modulate the root forging response at LN condition.
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DOI：
10.15252/embj.2021108664
发表时间：
2022-02-01
期刊：
The EMBO journal
影响因子：
--
作者：
Albertos P;Dündar G;Schenk P;Carrera S;Cavelius P;Sieberer T;Poppenberger B
通讯作者：
Poppenberger B
影响因子：
7.4
作者：
Giehl, Ricardo F. H.;von Wiren, Nicolaus
通讯作者：
von Wiren, Nicolaus
影响因子：
16
作者：
Kim TW;Guan S;Burlingame AL;Wang ZY
通讯作者：
Wang ZY
影响因子：
56.9
作者：
He, JX;Gendron, JM;Wang, ZY
通讯作者：
Wang, ZY
影响因子：
64.5
作者：
Li, J;Wen, JQ;Walker, JC
通讯作者：
Walker, JC