Acetate transport and utilization in the rat brain.
Acetate transport and utilization in the rat brain.
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DOI：
10.1111/j.1471-4159.2009.05895.x
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发表时间：
2009-05
影响因子：
4.7
通讯作者：
Henry PG
中科院分区：
文献类型：
--
作者：
Deelchand DK;Shestov AA;Koski DM;Uğurbil K;Henry PG
醋酸酯是一种胶质特异性底物，是研究神经元-胶质相互作用的有吸引力的葡萄糖替代品。本研究采用核磁共振波谱法研究了[2-13C]乙酸在体内大鼠脑内的摄取和利用动力学。随着血浆醋酸盐浓度的升高，脑醋酸盐利用率（CMRace）逐渐升高，在血浆醋酸盐浓度bb0 ~ 3mm时接近饱和，而脑醋酸盐浓度继续升高。脑内醋酸盐利用的Michaelis-Menten常数（）远小于通过血脑屏障的醋酸盐运输的Michaelis-Menten常数（）。醋酸盐通过血脑屏障的最大运输能力（）是脑内醋酸盐最大利用率（）的近两倍。我们得出结论，在我们的实验条件下，当血浆乙酸浓度增加到2-3 mM以上时，脑乙酸利用达到饱和。在如此高的血浆乙酸浓度下，胶质乙酸代谢的限速步骤不是血脑屏障，而是在乙酸进入脑后发生的。
Acetate, a glial-specific substrate, is an attractive alternative to glucose for the study of neuronal-glial interactions. The present study investigates the kinetics of acetate uptake and utilization in the rat brain in vivo during infusion of [2-13C]acetate using NMR spectroscopy. When plasma acetate concentration was increased, the rate of brain acetate utilization (CMRace) increased progressively and reached close to saturation for plasma acetate concentration > 2-3 mM, whereas brain acetate concentration continued to increase. The Michaelis-Menten constant for brain acetate utilization ( ) was much smaller than for acetate transport through the blood-brain barrier ( ). The maximum transport capacity of acetate through the blood-brain barrier ( ) was nearly two-fold higher than the maximum rate of brain acetate utilization ( ). We conclude that, under our experimental conditions, brain acetate utilization is saturated when plasma acetate concentrations increase above 2-3 mM. At such high plasma acetate concentration, the rate-limiting step for glial acetate metabolism is not the blood-brain barrier, but occurs after entry of acetate into the brain.
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影响因子：
7.4
作者：
de Graaf, RA;Behar, KL
通讯作者：
Behar, KL
影响因子：
3.3
作者：
Henry, PG;Marjanska, M;Ugurbil, K
通讯作者：
Ugurbil, K
DOI：
10.1097/00004647-200105000-00002
发表时间：
2001-05-01
影响因子：
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作者：
de Graaf, RA;Pan, JW;Rothman, DL
通讯作者：
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
2.9
作者：
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Ross, BD