T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs.
T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs.
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慢性丙型肝炎患者的T细胞受体β链和B细胞受体曲目共存HBSAG和抗HBS。
DOI：
10.3390/pathogens11070727
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发表时间：
2022-06-26
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影响因子：
--
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对抗原作出反应的抗体与T细胞和B细胞受体的免疫系统有关。然而，一些慢性乙型肝炎(CHB)患者同时存在不能中和乙肝病毒的HBs Ag和抗-HBs抗体(抗-HBs)。我们试图调查在慢性乙肝患者中产生这种反应的谱系。用MiXCR分析外周血基因组β的T细胞受体DNA链(Trb)和B细胞受体(Bcr)谱带。用clusTCR进行T细胞受体(TCR)的聚类分析，用多个Em选择基序进行模体激发(Meme)。入选对象76例，其中乙肝病毒表面抗原与抗-HBs共存的慢性乙肝患者26例(DP组)，抗-HBs单项阳性的健康体检者25例(SP组)，CHB患者25例(CHB组)。39、90岁组bcr克隆长度差异有统计学意义(p=0.005、0.036)。“CASSLG”基序在DP组显著高于SP组和CHB组，可能与共存有关；“GAGPLT”基序仅在SP组出现，可能与抗-Hb表达有关。这些为疫苗开发和慢性乙肝治疗提供了重要的见解。
Antibodies in response to antigens are related to the immune repertoire of T- and B-cell receptors. However, some patients with chronic hepatitis B (CHB) have coexisting HBsAg and anti-HBsAg antibodies (anti-HBs) that cannot neutralize HBV. We attempted to investigate the repertoires that produce this response in CHB patients. The T-cell receptor β chain (TRB) and B-cell receptor (BCR) repertoires of peripheral blood genomic DNA were analyzed using MiXCR. T-cell receptor (TCR) cluster analysis was carried out by clusTCR, and motifs prediction was selected by Multiple Em for Motif Elicitation (MEME). A total of 76 subjects were enrolled, including 26 HBsAg and anti-HBs coexisting patients with CHB (DP group), 25 anti-HBs single-positive healthy people (SP group), and 25 CHB patients (CHB group). The clone length of BCR in 39, 90 was significantly different among these groups (p = 0.005, 0.036). The motif “CASSLG” in the DP group was significantly higher than SP and CHB groups and may relate to coexistence, and the motif “GAGPLT” was only shown in the SP group and may relate to anti-HB expression. These provide important insights into vaccine development and CHB treatment.
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影响因子：
5.8
作者：
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14.9
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Bailey TL;Johnson J;Grant CE;Noble WS
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Noble WS
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