Relative Abundance of Lipid Metabolites in Spermatozoa across Three Compartments.
Relative Abundance of Lipid Metabolites in Spermatozoa across Three Compartments.
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DOI：
10.3390/ijms231911655
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发表时间：
2022-10-01
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
男性生育能力，如精子的数量和前向运动能力，受到肥胖、血脂异常和代谢疾病的负面影响。然而，脂质在精子中的相对分布以及为精子发生提供脂质的两个隔室（精液和血清）尚未研究。我们假设血清和精液中脂质的变化可能影响精子的脂质组成和前向运动能力。在NHS诊所招募了60名年龄35岁（中位数（范围20-45））和BMI 30.4 kg/m2（24-36.5）的男性，进行生育力低下的初步调查。男性提供血清和精液样本，按照严格的验收标准进行精子计数和活力分析。将血清（n = 60）、精子（n = 26）和精液（n = 60）冷冻用于批量脂质组学分析。精子和精液的脂质组成相当，但与血清脂质组有显着差异。精子表现出高丰度的神经酰胺、极长链脂肪酸（C20-22）和某些磷脂（鞘磷脂、缩醛磷脂、磷脂酰乙醇胺），低丰度的磷脂酰胆碱、胆固醇和甘油三酯。精子前向运动能力低的男性有证据表明，血清和精子室之间的许多脂质物质的浓度梯度较小。精子富含多种脂质物质，这些脂质物质可能有助于关键的细胞功能。脂质代谢显示精子前向运动能力降低的男性间室调节减少。
Male fertility, as manifest by the quantity and progressive motility of spermatozoa, is negatively impacted by obesity, dyslipidaemia and metabolic disease. However, the relative distribution of lipids in spermatozoa and the two compartments which supply lipids for spermatogenesis (seminal fluid and blood serum) has not been studied. We hypothesised that altered availability of lipids in blood serum and seminal fluid may affect the lipid composition and progressive motility of sperm. 60 men of age 35 years (median (range 20–45) and BMI 30.4 kg/m2 (24–36.5) under preliminary investigation for subfertility were recruited at an NHS clinic. Men provided samples of serum and semen, subject to strict acceptance criteria, for analysis of spermatozoa count and motility. Blood serum (n = 60), spermatozoa (n = 26) and seminal fluid (n = 60) were frozen for batch lipidomics analysis. Spermatozoa and seminal fluid had comparable lipid composition but showed marked differences with the serum lipidome. Spermatozoa demonstrated high abundance of ceramides, very-long-chain fatty acids (C20-22), and certain phospholipids (sphingomyelins, plasmalogens, phosphatidylethanolamines) with low abundance of phosphatidylcholines, cholesterol and triglycerides. Men with spermatozoa of low progressive motility had evidence of fewer concentration gradients for many lipid species between blood serum and spermatozoa compartments. Spermatozoa are abundant in multiple lipid species which are likely to contribute to key cellular functions. Lipid metabolism shows reduced regulation between compartments in men with spermatozoa with reduced progressive motility.
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影响因子：
4.5
作者：
Messias MCF;Mecatti GC;Priolli DG;de Oliveira Carvalho P
通讯作者：
de Oliveira Carvalho P
影响因子：
5.3
作者：
Kawano N;Yoshida K;Miyado K;Yoshida M
通讯作者：
Yoshida M
影响因子：
4.5
作者：
Ferramosca, A.;Moscatelli, N.;Zara, V.
通讯作者：
Zara, V.
影响因子：
5.2
作者：
Condorelli RA;La Vignera S;Mongioì LM;Alamo A;Calogero AE
通讯作者：
Calogero AE
影响因子：
4.8
作者：
Dawaliby, Rosie;Trubbia, Cataldo;Govaerts, Cedric
通讯作者：
Govaerts, Cedric