Micromonospora zeae sp. nov., a novel endophytic actinomycete isolated from corn root (Zea mays L.)

Micromonospora zeae sp. nov., a novel endophytic actinomycete isolated from corn root (Zea mays L.)
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玉米小单孢菌 sp.

DOI:
10.1038/ja.2014.54
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发表时间:
2014-05
期刊:
The Journal of Antibiotics.
影响因子:
--
通讯作者:
Wensheng Xiang
Wensheng Xiang
中科院分区:
其他
文献类型:
--
作者:
Feiyu Jia;Lingyu Yan;, Deguang Yang;Wensheng Xiang

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从玉米根中分离到一株新的放线菌,命名为neau-gq9T。并使用多相方法进行表征。该菌具有小单孢菌属典型的形态和化学分类特征。根据16S rRNA基因序列的相似性分析,菌株neau-gq9T与扎莫尔小单孢菌CR38T(99.3%)、简隆小单孢菌neau-GRX11T(99.2%)、盐生小单孢菌Lupac 09T(99.2%)、乔氏小单孢菌2-19(6)T(98.9%)、共生小单孢菌2-30-b(28)T(98.6%)和卢帕氏小单孢菌14N(98.6%)的亲缘关系最近。基于16S rRNA基因和gyrB基因的系统发育分析表明,新菌株neau-gq9T是小单孢菌属的一员,与丛枝杆菌CR38T、jinlong gensis neau-GRX11 T、盐生乳杆菌Lupac 09 T、乔科里分枝杆菌2-19(6)T和乳杆菌Lupini Lupac 14N T的亲缘关系最近,结合DNA-DNA杂交、形态和生理特征,可以很容易地将新菌株与最近的亲缘关系区分开来。因此,提出菌株neau-gq9T是小单孢菌属的一个新种,命名为玉米小单孢菌。11月是提议的。模式株为NEAU-GQ9T(=CGMCC 4.7092 T=DSM 45882 T)。
A novel actinomycete, designated strain NEAU-gq9 T, was isolated from corn root (Zea mays L.) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-gq9 T was most closely related to Micromonospora zamorensis CR38 T (99.3%), Micromonospora jinlongensis NEAU-GRX11 T (99.2%), Micromonospora saelicesensis Lupac 09 T (99.2%), Micromonospora chokoriensis 2-19 (6) T (98.9%), Micromonospora coxensis 2-30-b (28) T (98.6%) and Micromonospora lupini Lupac 14N T (98.5%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-gq9 T is a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38 T, M. jinlongensis NEAU-GRX11 T, M. saelicesensis Lupac 09 T, M. chokoriensis 2-19 (6) T and M. lupini Lupac 14N T. A combination of DNA–DNA hybridization, morphological and physiological characteristics indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-gq9 T represents a novel species of the genus Micromonospora, for which the name Micromonospora zeae sp. nov. is proposed. The type strain is NEAU-gq9 T (= CGMCC 4.7092 T= DSM 45882 T).
DOI: 10.1111/j.1432-1033.1970.tb00830.x
发表时间: 1970
期刊: European journal of biochemistry
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