IGF-1 TMJ injections enhance mandibular growth and bone quality in juvenile rats.
IGF-1 TMJ injections enhance mandibular growth and bone quality in juvenile rats.
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IGF-1 TMJ注射可增强青少年大鼠的下颌生长和骨质。
DOI：
10.1111/ocr.12524
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发表时间：
2022-05
影响因子：
3.1
通讯作者：
Kapila S
中科院分区：
文献类型：
--
作者：
Joshi AS;Hatch NE;Hayami T;Jheon A;Kapila S
下颌骨发育不良的牙面矫形治疗具有不可预测的骨骼结局。尽管已知包括胰岛素样生长因子1（IGF-1）在内的几种生物调节剂有助于软骨细胞增殖，但它们在调节下颌骨生长方面的功效尚未得到验证。本研究的目的是确定局部递送IGF-1对幼年大鼠下颌骨生长和髁突骨质量/数量的影响。使用24只35日龄雄性Sprague-Dawley大鼠的机构动物园。将PBS或40 μg/kg（低剂量）IGF-1或80 μg/kg（高剂量）IGF-1以每周间隔双侧注射到大鼠的颞下颌关节中，持续四周。头影测量和微型计算机断层扫描测量被用来确定下颌骨的尺寸。骨和组织的矿物质密度，体积分数和矿物质含量进行了测定，血清IGF-1浓度测定。与对照组和低剂量IGF-1治疗的动物相比，关节内给予高剂量IGF-1导致下颌骨体和髁突长度显著增加6-12%。此外，IGF-1治疗导致下切牙与下颌平面的成角显著减少。相对于对照组和低剂量IGF-1组，高剂量IGF-1组的髁突骨体积、骨体积分数、矿物质含量和矿物质密度显著增加。所有组之间的血清IGF-1水平相似，证实局部给药的IGF-1全身暴露有限。局部给予高剂量80 μg/kg IGF-1可促进生长期大鼠下颌骨的生长和髁突骨的质量和数量。这一发现对调节下颌骨生长和潜在地增强髁突骨的健康和完整性具有意义。
Dentofacial orthopedic treatment of mandibular hypoplasia has unpredictable skeletal outcomes. Although several biomodulators including insulin-like growth factor 1 (IGF-1) are known to contribute to chondrocyte proliferation, their efficacy in modulating mandibular growth has not been validated. The aim of this study was to determine the effect of locally delivered IGF-1 on mandibular growth and condylar bone quality/quantity in juvenile rats. Institutional vivarium using twenty-four 35-day-old male Sprague-Dawley rats. PBS or 40 μg/kg (low-dose) IGF-1 or 80 μg/kg (high-dose) IGF-1 was injected bilaterally into the temporomandibular joints of the rats at weekly intervals for four weeks. Cephalometric and micro-computed tomography measurements were used to determine mandibular dimensions. Bone and tissue mineral density, volume fraction, and mineral content were determined, and serum IGF-1 concentrations assayed. Intra-articular administration of high-dose IGF-1 contributed to a significant 6-12% increase in mandibular body and condylar length compared to control and low-dose IGF-1 treated animals. Additionally, IGF-1 treatment resulted in a significant decrease in the angulation of the lower incisors to mandibular plane. Condylar bone volume, bone volume fraction, mineral content, and mineral density were significantly increased with high-dose IGF-1 relative to control and low-dose IGF-1 groups. Serum IGF-1 levels were similar between all groups confirming limited systemic exposure to the locally administered IGF-1. Local administration of high-dose 80 μg/kg IGF-1 enhances mandibular growth and condylar bone quality and quantity in growing rats. The findings have implications for modulating mandibular growth and potentially enhancing condylar bone health and integrity.
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影响因子：
3
作者：
Fuentes, MA;Opperman, LA;Hinton, RJ
通讯作者：
Hinton, RJ
影响因子：
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作者：
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TUCKER, MR
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影响因子：
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作者：
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通讯作者：
Athiviraham A