Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano.
Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano.
复制标题
比较转录组分析揭示了参与藤壶巨巴拉努斯火山幼虫定居的候选基因和途径
DOI：
10.3390/ijms18112253
复制
发表时间：
2017-10-27
影响因子：
5.6
通讯作者：
He L
中科院分区：
文献类型：
--
作者：
Yan G;Zhang G;Huang J;Lan Y;Sun J;Zeng C;Wang Y;Qian PY;He L
巨藤壶是海洋生物污损研究的模式生物之一。然而，由于缺乏基因组信息，进一步阐明幼虫定居的分子机制受到阻碍。本研究构建了巨藤壶幼虫定居的关键阶段--Cyprids和成虫的cDNA文库。经过高通量测序和从头组装，获得了42，620个unigenes，N50值为1532 bp。通过针对NCBI非冗余（nr）、Swiss-Prot、Cluster of Orthopathic Groups（COG）和京都基因和基因组百科全书（KEGG）数据库进行blasting来注释这些单基因。最终，分别鉴定出19，522、15，691、14，459和10，914个单基因。在两个阶段之间鉴定出22，158个差异表达基因（DEG）。与成虫期相比，8241个unigenes表达下调，13,917个unigenes表达上调。神经活性配体-受体相互作用途径（ko 04080）通过DEG的KEGG富集分析而显著富集，表明其可能参与幼虫定居。三个保守的allatostatin神经肽-受体对和两个光敏视蛋白的潜在功能进行了进一步的表征，表明它们可能调节Cyprin阶段的附着和变态。这些结果为藤壶幼虫定居的分子机制提供了更深层次的认识。
Megabalanus barnacle is one of the model organisms for marine biofouling research. However, further elucidation of molecular mechanisms underlying larval settlement has been hindered due to the lack of genomic information thus far. In the present study, cDNA libraries were constructed for cyprids, the key stage for larval settlement, and adults of Megabalanus volcano. After high-throughput sequencing and de novo assembly, 42,620 unigenes were obtained with a N50 value of 1532 bp. These unigenes were annotated by blasting against the NCBI non-redundant (nr), Swiss-Prot, Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Finally, 19,522, 15,691, 14,459, and 10,914 unigenes were identified correspondingly. There were 22,158 differentially expressed genes (DEGs) identified between two stages. Compared with the cyprid stage, 8241 unigenes were down-regulated and 13,917 unigenes were up-regulated at the adult stage. The neuroactive ligand-receptor interaction pathway (ko04080) was significantly enriched by KEGG enrichment analysis of the DEGs, suggesting that it possibly involved in larval settlement. Potential functions of three conserved allatostatin neuropeptide-receptor pairs and two light-sensitive opsin proteins were further characterized, indicating that they might regulate attachment and metamorphosis at cyprid stage. These results provided a deeper insight into the molecular mechanisms underlying larval settlement of barnacles.
登录
查看更多内容
影响因子：
3.7
作者：
Chen ZF;Matsumura K;Wang H;Arellano SM;Yan X;Alam I;Archer JA;Bajic VB;Qian PY
通讯作者：
Qian PY
DOI：
10.1073/pnas.1220285110
发表时间：
2013-05-14
影响因子：
11.1
作者：
Conzelmann, Markus;Williams, Elizabeth A.;Jekely, Gaspar
通讯作者：
Jekely, Gaspar
影响因子：
4.6
作者：
Hentze JL;Carlsson MA;Kondo S;Nässel DR;Rewitz KF
通讯作者：
Rewitz KF
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
4.6
作者：
Li Q;Lei S;Du K;Li L;Pang X;Wang Z;Wei M;Fu S;Hu L;Xu L
通讯作者：
Xu L