RNA-seq based transcriptomic analysis uncovers α-linolenic acid and jasmonic acid biosynthesis pathways respond to cold acclimation in Camellia japonica.
RNA-seq based transcriptomic analysis uncovers α-linolenic acid and jasmonic acid biosynthesis pathways respond to cold acclimation in Camellia japonica.
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DOI：
10.1038/srep36463
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发表时间：
2016-11-07
影响因子：
4.6
通讯作者：
Xu L
中科院分区：
文献类型：
--
作者：
Li Q;Lei S;Du K;Li L;Pang X;Wang Z;Wei M;Fu S;Hu L;Xu L
山茶花是一种著名的观赏花卉，原产于亚洲东南亚，包括日本、韩国、华南等地区。然而，茶花属的大多数物种对寒冷敏感。为了阐明山茶花植物的冷胁迫反应，我们对山茶花耐冷品种“江雪”进行了深度转录组测序，利用Illumina测序技术生成了约10.06亿条干净读数。干净读段的组装产生了 367,620 个转录本，其中包括 207,592 个 unigene。总体而言，在冷驯化过程中鉴定出了 28,038 个差异表达基因。对转录因子、蛋白激酶和植物激素信号相关基因反应的详细阐述描述了信号的相互作用，使植物能够微调冷胁迫反应。基于不饱和脂肪酸生物合成和茉莉酸生物合成相关基因的全局基因调控，推测不饱和脂肪酸生物合成和茉莉酸生物合成途径参与了粳稻的低温响应。这些结果得到了脂肪酸组成和茉莉酸含量测定的支持。我们的结果提供了对山茶花植物冷驯化反应的遗传和分子基础的见解。
Camellia is a well-known ornamental flower native to Southeast of Asia, including regions such as Japan, Korea and South China. However, most species in the genus Camellia are cold sensitive. To elucidate the cold stress responses in camellia plants, we carried out deep transcriptome sequencing of ‘Jiangxue’, a cold-tolerant cultivar of Camellia japonica, and approximately 1,006 million clean reads were generated using Illumina sequencing technology. The assembly of the clean reads produced 367,620 transcripts, including 207,592 unigenes. Overall, 28,038 differentially expressed genes were identified during cold acclimation. Detailed elucidation of responses of transcription factors, protein kinases and plant hormone signalling-related genes described the interplay of signal that allowed the plant to fine-tune cold stress responses. On the basis of global gene regulation of unsaturated fatty acid biosynthesis- and jasmonic acid biosynthesis-related genes, unsaturated fatty acid biosynthesis and jasmonic acid biosynthesis pathways were deduced to be involved in the low temperature responses in C. japonica. These results were supported by the determination of the fatty acid composition and jasmonic acid content. Our results provide insights into the genetic and molecular basis of the responses to cold acclimation in camellia plants.
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