Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae).
Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae).
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DOI：
10.1007/s11032-015-0220-x
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Gatehouse AMR
中科院分区：
文献类型：
--
作者：
Guan W;Ferry N;Edwards MG;Bell HA;Othman H;Gatehouse JA;Gatehouse AMR
麦长管蚜Sitobion avenae（F.）是小麦的主要害虫，作为病毒载体以及造成直接植物损害。英国常见的小麦品种对这种害虫只有有限的抵抗力。本研究旨在研究一个二倍体小麦品系（ACC 20 PGR 1755）的抗病潜力。燕麦，作为抗性基因的来源。二倍体小麦品系被证实为部分抗性，显著降低了蚜虫的繁殖力、寿命和生长速率。蛋白质组学分析表明，大约200个蛋白质点可重复检测叶片提取物从两个抗性线和一个相当的敏感线（ACC 5 PGR 1735），使用二维凝胶电泳和图像比较软件。在蚜虫取食24 h后，抗性品系中有24个点显著上调（>2倍）（分别有13个和11个涉及局部和全身反应）。通过数据库搜索与MS和MS/MS数据的组合鉴定了大约50%的所有差异表达蛋白质点，揭示了大多数被蚜虫侵染上调的蛋白质参与代谢过程（包括光合作用）和转录调控。然而，仅在抗性品系中，几种应激反应蛋白（包括NBS-LRR-like蛋白）和氧化应激反应蛋白被鉴定为响应蚜虫进食而上调，以及参与DNA合成/复制/修复的蛋白。本研究表明，抗蚜二倍体材料ACC 20 PGR 1755可为小麦抗蚜育种提供有价值的资源。本文的在线版本（doi：10.1007/s11032-015-0220-x）包含补充材料，可供授权用户使用。
The grain aphid Sitobion avenae (F.) is a major pest of wheat, acting as a virus vector as well as causing direct plant damage. Commonly grown wheat varieties in the UK have only limited resistance to this pest. The present study was carried out to investigate the potential of a diploid wheat line (ACC20 PGR1755), reported as exhibiting resistance to S. avenae, to serve as a source of resistance genes. The diploid wheat line was confirmed as partially resistant, substantially reducing the fecundity, longevity and growth rate of the aphid. Proteomic analysis showed that approximately 200 protein spots were reproducibly detected in leaf extracts from both the resistant line and a comparable susceptible line (ACC5 PGR1735) using two-dimensional gel electrophoresis and image comparison software. Twenty-four spots were significantly up-regulated (>2-fold) in the resistant line after 24 h of aphid feeding (13 and 11 involved in local and systemic responses, respectively). Approximately 50 % of all differentially expressed protein spots were identified by a combination of database searching with MS and MS/MS data, revealing that the majority of proteins up-regulated by aphid infestation were involved in metabolic processes (including photosynthesis) and transcriptional regulation. However, in the resistant line only, several stress response proteins (including NBS–LRR-like proteins) and oxidative stress response proteins were identified as up-regulated in response to aphid feeding, as well as proteins involved in DNA synthesis/replication/repair. This study indicates that the resistant diploid line ACC20 PGR1755 may provide a valuable resource in breeding wheat for resistance to aphids. The online version of this article (doi:10.1007/s11032-015-0220-x) contains supplementary material, which is available to authorized users.
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