Differential proteomic analysis of Arabidopsis thaliana genotypes exhibiting resistance or susceptibility to the insect herbivore, Plutella xylostella.
Differential proteomic analysis of Arabidopsis thaliana genotypes exhibiting resistance or susceptibility to the insect herbivore, Plutella xylostella.
复制标题
DOI：
10.1371/journal.pone.0010103
复制
发表时间：
2010-04-08
期刊：
影响因子：
3.7
通讯作者：
Tomsett AB
中科院分区：
文献类型：
--
作者：
Collins RM;Afzal M;Ward DA;Prescott MC;Sait SM;Rees HH;Tomsett AB
采用蛋白质组学方法研究了小菜蛾（Plutella xylostella）幼虫对草食敏感和抗性拟南芥摄食行为的生理影响因素。对162个重组自交系（Rils）的叶片进行筛选，以确定小菜蛾幼虫对小菜蛾抗性最少或敏感最多的基因型。2D-PAGE显示，在鉴定出的耐药和易感ril之间，蛋白质组学存在显著差异。蛋白质组学结果，以及抗性核糖核酸中过氧化氢产量增加的检测，表明小菜蛾抗性与活性氧（ROS）（特别是H2O2）水平增加之间存在相关性，并且这在草食之前就表达了。许多在抗菜蛾的Rils中更丰富的蛋白质在其他生物系统中已知参与限制ROS损伤。这些蛋白包括碳酸酐酶、苹果酸脱氢酶、谷胱甘肽s转移酶、异柠檬酸脱氢酶样蛋白（R1）和脂酰胺脱氢酶。此外，发芽样蛋白3异构体的模式也可能表明抗性ril中活性氧含量较高。在抗氧化基因中发生更大的氧化应激，与此相一致的是，在敏感基因中观察到更多的光合进化氧复合物多肽，这些多肽在氧化应激下被破坏。综合结果表明，活性氧的增加可能是拟南芥抗小菜蛾的主要机制，但这需要进一步的研究。
A proteomic study was conducted to investigate physiological factors affecting feeding behaviour by larvae of the insect, Plutella xylostella, on herbivore-susceptible and herbivore-resistant Arabidopsis thaliana. The leaves of 162 recombinant inbred lines (Rils) were screened to detect genotypes upon which Plutella larvae fed least (P. xylostella-resistant) or most (P. xylostella-susceptible). 2D-PAGE revealed significant differences in the proteomes between the identified resistant and susceptible Rils. The proteomic results, together with detection of increased production of hydrogen peroxide in resistant Rils, suggest a correlation between P. xylostella resistance and the production of increased levels of reactive oxygen species (ROS), in particular H2O2, and that this was expressed prior to herbivory. Many of the proteins that were more abundant in the Plutella-resistant Rils are known in other biological systems to be involved in limiting ROS damage. Such proteins included carbonic anhydrases, malate dehydrogenases, glutathione S-transferases, isocitrate dehydrogenase-like protein (R1), and lipoamide dehydrogenase. In addition, patterns of germin-like protein 3 isoforms could also be indicative of higher levels of reactive oxygen species in the resistant Rils. Consistent with the occurrence of greater oxidative stress in the resistant Rils is the observation of greater abundance in susceptible Rils of polypeptides of the photosynthetic oxygen-evolving complex, which are known to be damaged under oxidative stress. The combined results suggest that enhanced production of ROS may be a major pre-existing mechanism of Plutella resistance in Arabidopsis, but definitive corroboration of this requires much further work.
登录
查看更多内容
影响因子：
3.5
作者：
Guo, Xiaomei;Stotz, Henrik U.
通讯作者：
Stotz, Henrik U.
影响因子：
6.5
作者：
Hancock, JT;Henson, D;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
4.3
作者：
ANGELINI, R;MANES, F;FEDERICO, R
通讯作者：
FEDERICO, R
影响因子：
2.9
作者：
Giavalisco, P;Nordhoff, E;Klose, J
通讯作者：
Klose, J
影响因子：
2.3
作者：
BI, JL;FELTON, GW
通讯作者：
FELTON, GW