Annexin A3 as a Marker Protein for Microglia in the Central Nervous System of Rats.
Annexin A3 as a Marker Protein for Microglia in the Central Nervous System of Rats.
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膜联蛋白 A3 作为大鼠中枢神经系统小胶质细胞的标记蛋白
DOI：
10.1155/2021/5575090
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发表时间：
2021
影响因子：
3.1
通讯作者：
Zou W
中科院分区：
文献类型：
--
作者：
Zhang Z;Li Z;Ma Z;Deng M;Xing M;Wu J;Jiang S;Wang Q;Guo Q;Zou W
脑实质小胶质细胞在脑生理和病理条件下具有不同的形态学特征;因此，可视化这些细胞是有用的，作为进一步研究脑实质小胶质细胞功能的一种手段。膜联蛋白A3（Annexin A3，ANXA 3）在小胶质细胞中表达，但其是否可作为小胶质细胞及其生理功能的标志蛋白尚不清楚。在这里，我们比较了由ANXA 3，分化簇11b（CD 11b）和离子化钙结合衔接分子1（Iba 1）标记的实质小胶质细胞的分布和形态，并测量了ANXA 3在非实质巨噬细胞（脑膜和血管周围巨噬细胞）中的表达。我们还研究了ANXA 3，CD 11b和Iba 1在体内和体外的时空表达以及ANXA 3在小胶质细胞中的细胞功能。我们证明，ANXA 3阳性细胞丰富，均匀分布在整个脑组织和脊髓的成年大鼠。ANXA 3标记的小胶质细胞的形态和分布与中枢神经系统（CNS）中小胶质细胞特异性标记物CD 11b和Iba 1标记的小胶质细胞的形态和分布非常相似。ANXA 3在小胶质细胞胞浆中表达，在活化的小胶质细胞中表达显著增加。ANXA 3在非实质巨噬细胞中几乎检测不到。同时，ANXA 3在中枢神经系统不同区域的蛋白和mRNA表达水平与CD 11b和Iba 1不同。此外，ANXA 3的敲低抑制了小胶质细胞的增殖和迁移，而ANXA 3的过表达增强了这些活动。本研究证实ANXA 3可能是成年大鼠CNS中实质小胶质细胞的新标志物，并丰富了我们对ANXA 3从表达模式到生理功能的理解。
The parenchymal microglia possess different morphological characteristics in cerebral physiological and pathological conditions; thus, visualizing these cells is useful as a means of further investigating parenchymal microglial function. Annexin A3 (ANXA3) is expressed in microglia, but it is unknown whether it can be used as a marker protein for microglia and its physiological function. Here, we compared the distribution and morphology of parenchymal microglia labeled by ANXA3, cluster of differentiation 11b (CD11b), and ionized calcium-binding adaptor molecule 1 (Iba1) and measured the expression of ANXA3 in nonparenchymal macrophages (meningeal and perivascular macrophages). We also investigated the spatiotemporal expression of ANXA3, CD11b, and Iba1 in vivo and in vitro and the cellular function of ANXA3 in microglia. We demonstrated that ANXA3-positive cells were abundant and evenly distributed throughout the whole brain tissue and spinal cord of adult rats. The morphology and distribution of ANXA3-labeled microglia were quite similar to those labeled by the microglial-specific markers CD11b and Iba1 in the central nervous system (CNS). ANXA3 was expressed in the cytoplasm of microglia, and its expression was significantly increased in activated microglia. ANXA3 was almost undetectable in the nonparenchymal macrophages. Meanwhile, the protein and mRNA expression levels of ANXA3 in different regions of the CNS were different from those of CD11b and Iba1. Moreover, knockdown of ANXA3 inhibited the proliferation and migration of microglia, while overexpression of ANXA3 enhanced these activities. This study confirms that ANXA3 may be a novel marker for parenchymal microglia in the CNS of adult rats and enriches our understanding of ANXA3 from expression patterns to physiological function.
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影响因子：
1.7
作者：
OGURA, K;OGAWA, M;YOSHIDA, M
通讯作者：
YOSHIDA, M
影响因子：
4.3
作者：
Dilshara, Matharage Gayani;Jayasooriya, Rajapaksha Gedara Prasad Tharanga;Kim, Gi-Young
通讯作者：
Kim, Gi-Young
影响因子：
13.5
作者：
Harry, G. Jean
通讯作者：
Harry, G. Jean
DOI：
10.1515/prilozi-2015-0062
发表时间：
2015-01-01
期刊：
Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki)
影响因子：
--
作者：
Stankov, Aleksandar;Belakaposka-Srpanova, Viktorija;Rosoklija, Gorazd
通讯作者：
Rosoklija, Gorazd
电针预处理通过 α7 烟碱乙酰胆碱受体介导的小胶质细胞表型转化减轻脑缺血损伤
影响因子：
5.3
作者：
Ma, Zhi;Zhang, Zengli;Wang, Qiang
通讯作者：
Wang, Qiang