Shortening velocity and ATPase activity of rat skeletal muscle fibers: effects of endurance exercise training.
Shortening velocity and ATPase activity of rat skeletal muscle fibers: effects of endurance exercise training.
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大鼠骨骼肌纤维的缩短速度和 ATP 酶活性：耐力运动训练的影响。
DOI：
10.1152/ajpcell.1994.266.6.c1699
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Fitts,RH
中科院分区：
文献类型：
--
作者：
Schluter,JM;Fitts,RH
通过对大鼠后肢肌单个皮肤纤维的力学性质的测量，验证了快型IIb纤维的最大缩短速度(Vo)高于快型IIa纤维的假设，并认为这种差异直接归因于IIb型纤维中的肌原纤维三磷酸腺苷酶(ATPase)活性较高。另外还进行了测量，以检验有规律的耐力运动分别增加和降低I型和IIa型纤维的Vo，以及改变的Vo与纤维ATPase活性的相应变化有关的假设。大鼠进行8~12wk跑台运动，每天跑2 h，5天/wk，以27m/min的速度上升15%。用松弛试验测定纤维Vo，用荧光法测定同一纤维中的ATPase。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测定各纤维的肌球蛋白同工酶。IIb型纤维的平均+/-SE Vo(7.9+/-0.22纤维长度/S)显著大于IIa型纤维(4.4+/-0.21纤维长度/S)，且Vo越高，ATPase活性越高(927+/-70vs.760+/-60微米·分-1 mm-3)。运动计划导致心肌肥厚，线粒体标志酶柠檬酸合成酶增加近两倍。运动对任何纤维类型的纤维直径和每横截面积的峰值张力都没有影响，但重要的是，它显著增加了(23%)比目鱼肌慢型I型纤维的Vo和ATPase活性。
Mechanical properties were measured in single skinned fibers from rat hindlimb muscle to test the hypothesis that the fast type IIb fiber exhibits a higher maximal shortening velocity (Vo) than the fast type IIa fiber and that the difference is directly attributable to a higher myofibrillar adenosinetriphosphatase (ATPase) activity in the type IIb fiber. Additional measurements were made to test the hypotheses that regular endurance exercise increases and decreases the Vo of the type I and IIa fiber, respectively, and that the altered Vo is associated with a corresponding change in the fiber ATPase activity. Rats were exercised by 8-12 wk of treadmill running for 2 h/day, 5 day/wk, up a 15% grade at a speed of 27 m/min. Fiber Vo was determined by the slack test, and the ATPase was measured fluorometrically in the same fiber. The myosin isozyme profile of each fiber was subsequently determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The mean +/- SE Vo (7.9 +/- 0.22 fiber lengths/s) of the type IIb fiber was significantly greater than the type IIa fiber (4.4 +/- 0.21 fiber lengths/s), and the higher Vo was associated with a higher ATPase activity (927 +/- 70 vs. 760 +/- 60 microM.min-1.mm-3). The exercise program induced cardiac hypertrophy and an approximately twofold increase in the mitochondrial marker enzyme citrate synthase. Exercise had no effect on fiber diameter or peak tension per cross-sectional area in any fiber type, but, importantly, it significantly increased (23%) both the Vo and the ATPase activity of the slow type I fiber of the soleus.(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
5.5
作者：
BARANY, M;CLOSE, RI
通讯作者：
CLOSE, RI
影响因子：
3.3
作者：
FITTS, RH;COSTILL, DL;GARDETTO, PR
通讯作者：
GARDETTO, PR
DOI：
10.1113/jphysiol.1981.sp013580
发表时间：
1981-02
期刊：
The Journal of Physiology
影响因子：
--
作者：
F. Julian;R. Moss
通讯作者：
F. Julian;R. Moss
耐力训练可引起大鼠膈肌肌球蛋白重链亚型的变化。
DOI：
10.2170/jjphysiol.40.759
发表时间：
1990
期刊：
The Japanese journal of physiology
影响因子：
--
作者：
T. Sugiura;A. Morimoto;Y. Sakata;T. Watanabe;N. Murakami
通讯作者：
N. Murakami
耐力训练诱导的人类骨骼肌纤维中收缩和调节蛋白的慢速和快速亚型共存。
DOI：
10.1111/j.1748-1716.1987.tb08216.x
发表时间：
1987
期刊：
Acta physiologica Scandinavica
影响因子：
--
作者：
P. Schantz;G. Dhoot
通讯作者：
G. Dhoot