Plasmodium falciparum genotypes diversity in symptomatic malaria of children living in an urban and a rural setting in Burkina Faso.
Plasmodium falciparum genotypes diversity in symptomatic malaria of children living in an urban and a rural setting in Burkina Faso.
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DOI：
10.1186/1475-2875-8-135
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发表时间：
2009-06-20
期刊：
影响因子：
3
通讯作者：
Sirima SB
中科院分区：
文献类型：
--
作者：
Soulama I;Nébié I;Ouédraogo A;Gansane A;Diarra A;Tiono AB;Bougouma EC;Konaté AT;Kabré GB;Taylor WR;Sirima SB
疟疾的临床表现被认为是寄生虫和人类遗传之间复杂相互作用的结果，在农村和城市地区有所不同。分析生活在这两个不同地区的非复杂性疟疾儿童的恶性疟原虫遗传多样性，可能有助于了解城市化对恶性疟原虫基因型分布的影响。通过巢式PCR扩增msp1和msp2基因，对分别生活在布基纳法索农村和城市地区的75名和89名无并发症疟疾感染儿童收集的分离物进行分析，比较恶性疟原虫的多样性。与其他msp1等位基因家族相比，K1等位基因家族在这两个地点的儿童中普遍存在（频率为90%）。msp1的mad20等位基因家族在城市（85.3%）比农村（63.2%）更普遍（p = 0.0001）。在城市地区，msp2的3D7等位基因比FC27等位基因更为普遍，其中3D7 300bp等位基因频率较高（约30%）。感染的多重性在城市地区为1至6，在农村地区为1至7。在多重感染的频率（p = 0.6）： 96.0% （95% ci: 91.6-100）的城市和93.1% （95% c）无差异。I: 87.6-98.6)。感染复杂程度随年龄增加而增加[p = 0.04（农村），p = 0.06（城市）]。部分等位基因家族（MAD20、FC27、3D7）存在城乡差异，城市化可能对恶性疟原虫遗传变异产生影响。在实施疟疾控制措施时应考虑到这一点。
The clinical presentation of malaria, considered as the result of a complex interaction between parasite and human genetics, is described to be different between rural and urban areas. The analysis of the Plasmodium falciparum genetic diversity in children with uncomplicated malaria, living in these two different areas, may help to understand the effect of urbanization on the distribution of P. falciparum genotypes. Isolates collected from 75 and 89 children with uncomplicated malaria infection living in a rural and an urban area of Burkina Faso, respectively, were analysed by a nested PCR amplification of msp1 and msp2 genes to compare P. falciparum diversity. The K1 allelic family was widespread in children living in the two sites, compared to other msp1 allelic families (frequency >90%). The MAD 20 allelic family of msp1 was more prevalent (p = 0.0001) in the urban (85.3%) than the rural area (63.2%). In the urban area, the 3D7 alleles of msp2 were more prevalent compared to FC27 alleles, with a high frequency for the 3D7 300bp allele (>30%). The multiplicity of infection was in the range of one to six in the urban area and of one to seven in the rural area. There was no difference in the frequency of multiple infections (p = 0.6): 96.0% (95% C.I: 91.6–100) in urban versus 93.1% (95%C.I: 87.6–98.6) in rural areas. The complexity of infection increased with age [p = 0.04 (rural area), p = 0.06 (urban area)]. Urban-rural area differences were observed in some allelic families (MAD20, FC27, 3D7), suggesting a probable impact of urbanization on genetic variability of P. falciparum. This should be taken into account in the implementation of malaria control measures.
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DOI：
10.4269/ajtmh.1995.52.81
发表时间：
1995-01-01
影响因子：
3.3
作者：
NTOUMI, F;CONTAMIN, H;MERCEREAUPUIJALON, O
通讯作者：
MERCEREAUPUIJALON, O
DOI：
10.1016/s0035-9203(99)90322-x
发表时间：
1999-02-01
影响因子：
2.2
作者：
Smith, T;Beck, HP;Tanner, M
通讯作者：
Tanner, M
影响因子：
6.4
作者：
Ariey, F;Hommel, D;Mercereau-Puijalon, O
通讯作者：
Mercereau-Puijalon, O
影响因子：
3.3
作者：
Peyerl-Hoffmann, G;Jelinek, T;von Sonnenburg, F
通讯作者：
von Sonnenburg, F
影响因子：
2.4
作者：
Roper, C;Richardson, W;Arnot, DE
通讯作者：
Arnot, DE