Dexamethasone promotes calcium pyrophosphate dihydrate crystal formation by articular chondrocytes.
Dexamethasone promotes calcium pyrophosphate dihydrate crystal formation by articular chondrocytes.
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DOI：
10.3899/jrheum.080528
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Rosenthal AK
中科院分区：
文献类型：
--
作者：
Fahey M;Mitton E;Muth E;Rosenthal AK
Calcium pyrophosphate dihydrate crystals (CPPD) are commonly found in osteoarthritic joints and correlate with a poor prognosis. Intra-articular corticosteroids, such as dexamethasone (Dxm), are commonly used therapies for osteoarthritis with or without CPPD deposition. Dxm has variable effects in mineralization models. We investigated the effects of Dxm on CPPD crystal formation in a well established tissue culture model. Porcine articular chondrocytes were incubated with ATP to generate CPPD crystals. Chondrocytes incubated with or without ATP were exposed to 1–100 nM Dxm in the presence of 45Ca. Mineralization was measured by 45Ca uptake in the cell layer. We also investigated the effect of Dxm on mineralization-regulating enzymes such as alkaline phosphatase, NTPPPH and transglutaminase. Dxm significantly increased ATP-induced mineralization by articular chondrocytes. While alkaline phosphatase and NTPPPH activities were unchanged by Dxm, transglutaminase activity increased in a clear dose responsive manner. Levels of factor XIIIA mRNA and protein were increased by Dxm, while type II Tgase protein was unchanged. Transglutaminase inhibitors suppressed Dxm-induced increases in CPPD crystal formation. These findings suggest a potential for Dxm to contribute to pathologic mineralization in cartilage and reinforce a central role for the transglutaminase enzymes in CPPD crystal formation.
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影响因子：
4.1
作者：
Johnson, TS;Scholfield, CI;Griffin, M
通讯作者：
Griffin, M
影响因子：
--
作者：
MITCHELL, PG;STRUVE, JA;CHEUNG, HS
通讯作者：
CHEUNG, HS
影响因子：
7
作者：
Derfus, BA;Camacho, NP;Rosenthal, AK
通讯作者：
Rosenthal, AK
影响因子：
27.4
作者：
LEDINGHAM, J;REGAN, M;DOHERTY, M
通讯作者：
DOHERTY, M
影响因子：
2.2
作者：
AUTEFAGE, A;ALVINERIE, M;TOUTAIN, PL
通讯作者：
TOUTAIN, PL