The protective effect of glycyrrhizic acid on renal tubular epithelial cell injury induced by high glucose.
The protective effect of glycyrrhizic acid on renal tubular epithelial cell injury induced by high glucose.
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DOI：
10.3390/ijms150915026
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发表时间：
2014-08-26
影响因子：
5.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Hou S;Zheng F;Li Y;Gao L;Zhang J
本研究的目的是利用肾小管上皮细胞系（NRK-52 E）来确定甘草酸（GA）对2型糖尿病肾病的有益作用。将细胞分为正常组（NG）、高糖组（HG）和处理组（HG + GA）。MTT法检测细胞增殖情况;使用流式细胞术进行细胞周期分析。模型驱动架构（MDA），活性氧（ROS）和超氧化物歧化酶（SOD）也进行了测定。电镜和组织学观察细胞超微结构的变化。采用免疫组化、免疫荧光和Western blotting检测AMPK、SIRT 1、Mn-SOD和TGF-β 1的磷酸化水平。实时荧光定量PCR（RT-qPCR）检测Mn-SOD和PPARγ共激活因子1α（PGC-1a）mRNA的表达。结果发现，高糖可促进NRK-52 E细胞增殖，促进TGF-β1表达，降低AMPK、SIRT 1和Mn-SOD的表达。这些影响显着衰减GA。我们的研究结果表明，GA对高糖诱导的细胞增殖和氧化应激的保护作用，至少部分通过增加AMPK，SIRT 1和Mn-SOD在NRK-52 E细胞中的表达。
The aim of this study was to determine the beneficial effect of glycyrrhizic acid (GA) on type 2 diabetic nephropathy using renal tubular epithelial cell line (NRK-52E). The cells are divided into normal group (NG), high glucose group (HG), and treatment group (HG + GA). The methylthiazoletetrazolium (MTT) assay was used to detect the cell proliferation. Cell cycle analysis was performed using flow cytometry. Model driven architecture (MDA), reactive oxygen species (ROS) and superoxide dismutase (SOD) were also measured. Electron microscopy and histological were used to detect the changes in cell ultrastructure. The phosphorylation of AMP-activated protein kinase (AMPK), silent information regulator T1 (SIRT1), manganese-superoxide dismutase (Mn-SOD) and transforming growth factor-β1 (TGF-β1) were assessed by immunohistochemistry, immunofluorescence, and western blotting. Real-time fluorescent quantitative PCR (RT-qPCR) was used to measure Mn-SOD and PPARγ co-activator 1α (PGC-1a) mRNA. We find that high glucose increases NRK-52E cell proliferation and TGF-β1 expression, but decreases expression of AMPK, SIRT1 and Mn-SOD. These effects are significantly attenuated by GA. Our findings suggest that GA has protective effects against high glucose-induced cell proliferation and oxidative stress at least in part by increasing AMPK, SIRT1 and Mn-SOD expression in NRK-52E cells.
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DOI：
10.2741/4047
发表时间：
2012-06-01
期刊：
Frontiers in bioscience (Landmark edition)
影响因子：
--
作者：
Tan B;Li X;Yin Y;Wu Z;Liu C;Tekwe CD;Wu G
通讯作者：
Wu G
影响因子：
2.1
作者：
Liang, Huiyun;Ward, Walter F.
通讯作者：
Ward, Walter F.
影响因子：
2
作者：
Lee, Chan-Ho;Park, Sang-Won;Lee, Sun-Mee
通讯作者：
Lee, Sun-Mee
影响因子：
--
作者：
Karadeniz A;Simsek N;Karakus E;Yildirim S;Kara A;Can I;Kisa F;Emre H;Turkeli M
通讯作者：
Turkeli M
影响因子：
9.8
作者：
Leone, TC;Lehman, JJ;Kelly, DP
通讯作者：
Kelly, DP